Sowmya S V, Augustine Dominic, Hemanth Beena, Prathab Arcot Gopal, Alamoudi Ahmed, Bahammam Hammam Ahmed, Bahammam Sarah Ahmed, Bahammam Maha A, Haragannavar Vanishri C, Prabhu Sonia, Patil Shankargouda
Department of Oral Pathology & Microbiology, Faculty of Dental Sciences, M.S. Ramaiah University of Applied Sciences, Bangalore 560054, Karnataka, India.
Department of Microbiology, Ramaiah Medical College, MSRIT Post, Bangalore 560054, Karnataka, India.
Microorganisms. 2022 Jun 15;10(6):1226. doi: 10.3390/microorganisms10061226.
In the wake of the COVID-19 pandemic, fungal infections of the maxillofacial region have become prevalent, making their accurate diagnosis vital. Histopathological staining remains a simple, cost-effective technique for differentiation and diagnosis of the causative fungal organisms. The present study aims to evaluate the staining efficacy of Periodic Acid-Schiff (PAS), Alcian Blue, Safranin-O and Gomori's Methenamine Silver (GMS) on fungal smears. This research work also attempts to study the morphometric characteristics of , , . , and , 10 smears each, were stained using PAS, Alcian Blue, Safranin-O and GMS. The morphological characteristics and staining efficacy were examined, and semi-quantitative scoring was performed. , and were stained for the first time with Safranin-O. The morphometric traits were then analyzed using an image analysis software. Safranin-O provided the most reliable staining efficacy amongst the stains and optimum morphological definition for all three organisms. Safranin-O was found to be superior to PAS and GMS, ensuring detection of even the most minute mycotic colonies. The hyphae of to be the largest, and the spores and fruiting body of were found to be the largest amongst the three organisms compared. Early and accurate diagnosis of fungal infections can significantly reduce morbidity in orofacial fungal infections.
在新冠疫情之后，颌面部真菌感染变得普遍，因此准确诊断至关重要。组织病理学染色仍然是区分和诊断致病性真菌的一种简单且经济高效的技术。本研究旨在评估过碘酸希夫（PAS）、阿尔辛蓝、番红O和高碘酸苏木精银（GMS）对真菌涂片的染色效果。这项研究工作还试图研究[此处原文缺失具体真菌名称]、[此处原文缺失具体真菌名称]和[此处原文缺失具体真菌名称]的形态计量学特征。分别用PAS、阿尔辛蓝、番红O和GMS对每种真菌的10张涂片进行染色。检查形态特征和染色效果，并进行半定量评分。[此处原文缺失具体真菌名称]、[此处原文缺失具体真菌名称]和[此处原文缺失具体真菌名称]首次用番红O染色。然后使用图像分析软件分析形态计量学特征。在所有染色剂中，番红O提供了最可靠的染色效果，并且对所有三种真菌都有最佳的形态清晰度。发现番红O优于PAS和GMS，能够检测到即使是最微小的霉菌菌落。在所比较的三种真菌中，[此处原文缺失具体真菌名称]的菌丝最大，[此处原文缺失具体真菌名称]的孢子和子实体最大。真菌感染的早期准确诊断可显著降低口腔面部真菌感染的发病率。
