Department of Nano-Bioengineering, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon 22012, Korea.
Molecules. 2022 Jun 13;27(12):3803. doi: 10.3390/molecules27123803.
We investigated whether Scutellaria barbata D. Don (Family Lamiaceae) (SBD), a traditional medicine used for heat clearing and detoxification, possesses antiphotoaging properties. Pretreatment of NIH-3T3 skin fibroblast cells with non-toxicological levels of water extract of SBD (WESBD) and ethanol extract of SBD (EESBD) restored the expression of procollagen type-1 (COL1A1), matrix metalloproteinase-1a (MMP-1a), interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemotactic protein-3 (MCP-3) genes following abnormal expression induced by ultraviolet B (UVB) irradiation. WESBD/EESBD administration to the dorsal skin area of hairless mice significantly (p < 0.05) inhibited UVB-induced wrinkle formation and epidermal thickness. The WESBD and EESBD treatments also restored the dermal collagen content, which was decreased by the UVB treatment, and normal COL1A1 and MMP-1a expression. Interestingly, both the WESBD and EESBD pretreatments significantly attenuated UVB-induced phosphorylation of protein kinase B (AKT) but not that of mitogen-activated protein kinases (MAPKs). This finding indicates that the antiphotoaging effects of WESBD and EESBD may be related to attenuation of UVB-induced overactivation of AKT phosphorylation. High performance liquid chromatography (HPLC) and mass spectrometry analysis revealed that isorhamentin and scutebarbatine I were major single components of EESBD. These results suggest that WESBD and EESBD may have potential in development as antiphotoaging agents.
我们研究了黄芩（唇形科）（SBD）是否具有抗光老化特性，黄芩是一种用于清热解毒的传统药物。用非毒性水平的 SBD 水提物（WESBD）和 SBD 乙醇提取物（EESBD）预处理 NIH-3T3 皮肤成纤维细胞，可恢复 COL1A1、MMP-1a、IL-6、IL-8 和单核细胞趋化蛋白-3（MCP-3）基因的异常表达，这些基因在 UVB 照射后表达异常。WESBD/EESBD 给药于无毛小鼠背部皮肤区域，可显著（p<0.05）抑制 UVB 诱导的皱纹形成和表皮增厚。WESBD 和 EESBD 处理还恢复了真皮胶原含量，该含量因 UVB 处理而降低，且正常的 COL1A1 和 MMP-1a 表达。有趣的是，WESBD 和 EESBD 预处理均显著减弱了 UVB 诱导的蛋白激酶 B（AKT）磷酸化，但对丝裂原活化蛋白激酶（MAPKs）的磷酸化无影响。这一发现表明，WESBD 和 EESBD 的抗光老化作用可能与减弱 AKT 磷酸化的过度激活有关。高效液相色谱（HPLC）和质谱分析表明，异甘草素和黄芩素 I 是 EESBD 的主要单一成分。这些结果表明，WESBD 和 EESBD 可能具有开发为抗光老化剂的潜力。
