Institute of Pharmacology of Natural Products and Clinical Pharmacology, Ulm University, 89081 Ulm, Germany.
Laboratoire PhytoChemia, Saguenay, QC G7J 1H4, Canada.
Molecules. 2022 Jun 17;27(12):3903. doi: 10.3390/molecules27123903.
Oleogum resins of the genus have been used in traditional medicines for centuries. More than 200 species exhibit highly variable phytochemical compositions. A novel highly selective, sensitive, accurate HPLC-MS/MS method was developed and validated to quantify five characteristic phytosteroids and furanosesquiterpenoids, namely ()-guggulsterone, ()-guggulsterone, curzerenone, furanoeudesma-1,3-diene, and myrrhone. The resulting contents and additionally GC analysis were used to classify and differentiate oleogum resins of the species , , , , , and , as well as unspecified resins. Interestingly, a sample from Ogaden, Ethiopia, comprised 446 ng/mg guggulsterones presumed to be unique to from the Indian subcontinent. However, from Ogaden differed considerably from in respect to guggulsterones isomer's ratio. Moreover, the cytotoxicity of extracts, essential oils, botanical drugs containing , and pure compounds against the epidermoid carcinoma A431, malignant melanoma RPMI-7951 and SK-MEL-28 cells was investigated in vitro. Thereby, especially extract and essential oil exhibited high cytotoxicity against skin cancer cells with IC of 2.9-10.9 µg/mL, but were less toxic to normal keratinocytes. In summary, oleogum resins and its phytochemicals warrant further investigation aiming at chemotaxonomical classification as well as application in skin cancer treatment.
数百年以来，没药属植物的油胶树脂一直被应用于传统药物中。超过 200 种没药属植物表现出高度变化的植物化学成分。建立并验证了一种新型高度选择性、高灵敏度、准确的 HPLC-MS/MS 方法，用于定量分析五种特征性植物甾醇和呋喃倍半萜烯，即（）-芝麻脂素、（）-芝麻脂素、莪术酮、呋喃莪术二烯和没药酮。所得含量和另外的 GC 分析用于对 、 、 、 、 和未指定的没药属植物的油胶树脂进行分类和区分。有趣的是，来自埃塞俄比亚奥加登的一个样本包含 446ng/mg 的芝麻脂素，推测其为来自印度次大陆的独特成分。然而，来自奥加登的没药与其他没药属植物在芝麻脂素异构体比例方面存在显著差异。此外，体外研究了 提取物、精油、含有 的植物药以及纯化合物对表皮癌细胞 A431、恶性黑色素瘤 RPMI-7951 和 SK-MEL-28 的细胞毒性。因此，特别是 提取物和 精油对皮肤癌细胞具有高细胞毒性，IC 为 2.9-10.9µg/mL，而对正常角质形成细胞的毒性较小。总之，没药属植物油胶树脂及其植物化学物质值得进一步研究，以实现化学分类学分类以及在皮肤癌治疗中的应用。
