University of Belgrade-Faculty of Chemistry, Chair of Analytical Chemistry, Center for Excellence for Molecular Food Sciences, Studentski Trg 12-16, 11158 Belgrade, Serbia.
Justus Liebig University Giessen, Institute of Nutritional Science, Chair of Food Science, and Interdisciplinary Research Center, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
Molecules. 2022 Jun 19;27(12):3933. doi: 10.3390/molecules27123933.
The high consumption of plant-based foods on a global scale has increased the number of adulterations in the food industry. Along with this, analytical approaches to fraud detection need to be further developed. A nontargeted effect-directed profiling by high-performance thin-layer chromatography hyphenated with five effect-directed assays (free radical scavenging assay, bioassay, and acetylcholinesterase, butyrylcholinesterase, and tyrosinase inhibition assays) and multi-imaging provided additional information on the antioxidative, antimicrobial, and enzyme inhibition activities for 18 apple and 18 grape juices from markets in Serbia and Germany. Bioactive zones of interest were eluted using an elution head-based interface and further characterized by electrospray ionization high-resolution mass spectrometry. The different profiles were evaluated chemometrically, and several compounds, which were characteristic of samples from different markets located in Serbia and Germany, were identified in apple juice (such as chlorogenic acid, phloridzin, epicatechin, and caffeic acid) and grape juice (such as chlorogenic acid, epicatechin, and quercetin). The developed rapid and simple method for the quality assessment of fruit juices coming from different (geographic) markets showed clear quality differences. Thus, it could be used to learn more about quality differences, to detect fraud in fruit juice production, and to verify the authenticity of the origin.
在全球范围内，植物性食品的高消耗增加了食品行业掺假的数量。因此，需要进一步开发欺诈检测的分析方法。通过高效薄层色谱与五种效应导向分析（自由基清除分析、生物分析以及乙酰胆碱酯酶、丁酰胆碱酯酶和酪氨酸酶抑制分析）和多成像联用的非靶向效应导向分析 profiling，为来自塞尔维亚和德国市场的 18 种苹果汁和 18 种葡萄汁提供了关于抗氧化、抗菌和酶抑制活性的额外信息。使用基于洗脱头的接口洗脱感兴趣的生物活性区域，并通过电喷雾电离高分辨率质谱进一步表征。不同的图谱通过化学计量学进行评估，并在苹果汁（如绿原酸、根皮苷、表儿茶素和咖啡酸）和葡萄汁（如绿原酸、表儿茶素和槲皮素）中鉴定出了一些特征性地来自塞尔维亚和德国不同市场的样品的化合物。该开发的用于评估来自不同（地理）市场的果汁质量的快速简单方法显示出明显的质量差异。因此，它可用于了解更多关于质量差异的信息，检测果汁生产中的欺诈行为，并验证原产国的真实性。
