Chair of Food Science, Institute of Nutritional Science, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
Department of Chromatography, Faculty of Chemistry, Maria Curie-Sklodowska University, Maria Curie-Sklodowska Sq. 3, 20031 Lublin, Poland.
Molecules. 2023 Mar 23;28(7):2893. doi: 10.3390/molecules28072893.
Two herbal plants, D. leaf/fruit and L. flower, well-known in traditional medicine systems, were investigated using a non-target effect-directed profiling. High-performance thin-layer chromatography (HPTLC) was combined with 11 different effect-directed assays, including two multiplex bioassays, for assessing their bioactivity. Individual active zones were heart-cut eluted for separation via an orthogonal high-performance liquid chromatography column to heated electrospray ionization high-resolution mass spectrometry (HPLC-HESI-HRMS) for tentative assignment of molecular formulas according to literature data. The obtained effect-directed profiles provided information on 2,2-diphenyl-1-picrylhydrazyl scavenging, antibacterial (against and ), enzyme inhibition (tyrosinase, α-amylase, β-glucuronidase, butyrylcholinesterase, and acetylcholinesterase), endocrine (agonists and antagonists), and genotoxic (SOS-Umu-C) activities. The main bioactive compound zones in leaf were tentatively assigned to be syringin, vanilloloside, salidroside, α-hederin, cuneataside E, botulin, and oleanolic acid, while salidroside and quinatic acids were tentatively identified in the fruit. Taraxerol, kaempherol-3-rutinoside, kaempferol-3-glucoside, quercetin-3-rutinoside, and octadecenoic acid were tentatively found in the flower. This straightforward hyphenated technique made it possible to correlate the biological properties of the herbs with possible compounds. The meaningful bioactivity profiles contribute to a better understanding of the effects and to more efficient food control and food safety.
两种草药，D. 叶/果和 L. 花，在传统医学体系中广为人知，采用非靶向效应导向分析方法进行了研究。采用高效薄层色谱（HPTLC）结合 11 种不同的效应导向分析方法，包括两种多重生物测定法，评估它们的生物活性。对单个活性区进行中心切割洗脱，通过正交高效液相色谱柱进行分离，然后通过加热电喷雾电离高分辨率质谱（HPLC-HESI-HRMS）进行分离，根据文献数据对分子公式进行初步归属。获得的效应导向图谱提供了 2,2-二苯基-1-苦基肼清除、抗菌（对 和 ）、酶抑制（酪氨酸酶、α-淀粉酶、β-葡萄糖醛酸酶、丁酰胆碱酯酶和乙酰胆碱酯酶）、内分泌（激动剂和拮抗剂）和遗传毒性（SOS-Umu-C）活性的信息。叶中的主要生物活性化合物区被初步归属为丁香苷、香草酸苷、红景天苷、α-常春藤苷、楔叶糖苷 E、博替尼和齐墩果酸，而在果实中则初步鉴定出红景天苷和醌酸。在 花中，初步鉴定出蒲公英萜醇、山柰酚-3-鼠李糖苷、山柰酚-3-葡萄糖苷、槲皮素-3-鼠李糖苷和十八碳烯酸。这种直接的连接技术使将草药的生物特性与可能的化合物相关联成为可能。有意义的生物活性图谱有助于更好地理解作用机制，并提高食品控制和食品安全的效率。
