Correia Alexandra, Alves Pedro, Fróis-Martins Ricardo, Teixeira Luzia, Vilanova Manuel
ICBAS-Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Rua Jorge Viterbo Ferreira, 4050-313 Porto, Portugal.
I3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 4200-135 Porto, Portugal.
Vaccines (Basel). 2022 Jun 10;10(6):925. doi: 10.3390/vaccines10060925.
is an obligate intracellular protozoan responsible for abortion and stillbirths in cattle. We previously developed a mucosal vaccination approach using membrane proteins and CpG adjuvant that conferred long-term protection against neosporosis in mice. Here, we have extended this approach by alternatively using the carbomer-based adjuvant Carbigen™ in the immunizing preparation. Immunized mice presented higher proportions and numbers of memory CD4 and CD8 T cells. Stimulation of spleen, lungs and liver leukocytes with parasite antigens induced a marked production of IFN-γ and IL-17A and, less markedly, IL-4. This balanced response was also evident in that both parasite-specific IgG1 and IgG2c were raised by immunization, together with specific intestinal IgA. Upon intraperitoneal infection with , immunized mice presented lower parasitic burdens than sham-immunized controls. In the infected immunized mice, memory CD4 T cells predominantly expressed T-bet and RORγt, and CD8 T cells expressing T-bet were found increased. While spleen, lungs and liver leukocytes of both immunized and sham-immunized infected animals produced high amounts of IFN-γ, only the cells from immunized mice responded with high IL-17A production. Since in cattle both IFN-γ and IL-17A have been associated with protective mechanisms against infection, the elicited cytokine profile obtained using Carbigen as adjuvant indicates that it could be worth exploring for bovine neosporosis vaccination.
是一种专性细胞内原生动物，可导致牛流产和死产。我们之前开发了一种使用膜蛋白和CpG佐剂的黏膜疫苗接种方法，该方法可在小鼠中提供针对新孢子虫病的长期保护。在此，我们通过在免疫制剂中交替使用基于卡波姆的佐剂Carbigen™扩展了这种方法。免疫的小鼠呈现出更高比例和数量的记忆性CD4和CD8 T细胞。用寄生虫抗原刺激脾脏、肺和肝白细胞可诱导显著产生IFN-γ和IL-17A，而IL-4的产生则不太明显。这种平衡反应还体现在免疫接种可同时提高寄生虫特异性IgG1和IgG2c以及特异性肠道IgA。在用[具体病原体名称未给出]进行腹腔感染后，免疫的小鼠比假免疫对照呈现出更低的寄生虫负荷。在感染的免疫小鼠中，记忆性CD4 T细胞主要表达T-bet和RORγt，并且发现表达T-bet的CD8 T细胞增加。虽然免疫和假免疫感染动物的脾脏、肺和肝白细胞都产生大量IFN-γ，但只有免疫小鼠的细胞对高IL-17A产生有反应。由于在牛中IFN-γ和IL-17A都与针对[具体病原体名称未给出]感染的保护机制相关，使用Carbigen作为佐剂获得的引发的细胞因子谱表明，它值得用于牛新孢子虫病疫苗接种的探索。
