Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering/Key Laboratory of Ministry of Education, Guizhou University, Guiyang 550025, China.
Viruses. 2022 May 24;14(6):1120. doi: 10.3390/v14061120.
The viromic profile of Polyscias balfouriana cv. Marginata, a perennial woody and ornamental plant, was determined using ribosomal RNA-depleted total RNA (rRNA-depleted totRNA) sequencing. Five viruses (i.e., polyscias mosaic virus, PoMV; one potential novel rhabdovirus; and three novel viruses of Betaflexiviridae and Closteroviridae) were detected and prevalence-surveyed in Hainan province, China. The genomes of polyscias capillovirus 1 (PCaV-1) and polyscias citrivirus 1 (PCiV-1) of family Betaflexiviridae were completed, and the genomes of polyscias crinivirus 1 (PCrV-1) of Closteroviridae were nearly completed lacking the 5′ and 3′ termini. PCaV-1 shares 68% genome nucleotide (nt) identity and 66% replicase (Rep) amino acid (aa) identity with homologues in apple stem grooving virus (ASGV). PCiV-1 shares 65% genome nt identity and 64% Rep aa identity with homologs in citrus leaf blotch virus (CLBV). Meeting the species demarcation criteria, PCaV-1 and PCiV-1 were considered to be new species in genera Capillovirus and Citrivirus, respectively. PCrV-1 shares high genome nt identity (62%), heat shock protein 70-like protein (HSP70h) and RNA-dependent RNA polymerase (RdRp) aa identity (78−80%) with homologues in tomato chlorosis virus (ToCV). We tentatively consider PCrV-1 to be an unclassified member of the Crinivirus genus. PoMV, PCaV-1, PCiV-1, and PCrV-1 are the prevalent viruses with >73% occurrence in the Xinglong Tropical Botanical Garden, Hainan, China.
利用核糖体 RNA 耗尽的总 RNA（rRNA-depleted totRNA）测序技术，对多年生木本观赏植物金边富贵竹的病毒组进行了分析。在中国海南，检测到并流行调查了 5 种病毒（即金边富贵竹花叶病毒、PoMV；一种潜在的新型杆状病毒；以及贝塔 Flexiviridae 和 Closteroviridae 科的 3 种新型病毒）。完成了贝塔 Flexiviridae 科的金边富贵竹 capillovirus 1（PCaV-1）和金边富贵竹 citrivirus 1（PCiV-1）的基因组，以及 Closteroviridae 科的金边富贵竹 crinivirus 1（PCrV-1）的近完成基因组，缺少 5'和 3'末端。PCaV-1 与苹果茎沟病毒（ASGV）的同源物在基因组核苷酸（nt）上有 68%的同一性，在复制酶（Rep）氨基酸（aa）上有 66%的同一性。PCiV-1 与柑橘叶斑病毒（CLBV）的同源物在基因组 nt 上有 65%的同一性，在 Rep aa 上有 64%的同一性。符合物种划分标准，PCaV-1 和 PCiV-1 分别被认为是 Capillovirus 和 Citrivirus 属的新种。PCrV-1 与番茄黄化曲叶病毒（ToCV）的同源物在基因组 nt 上有 62%的同一性，在热休克蛋白 70 样蛋白（HSP70h）和 RNA 依赖性 RNA 聚合酶（RdRp）aa 上有 78-80%的同一性。我们暂将 PCrV-1 视为 Crinivirus 属的未分类成员。PoMV、PCaV-1、PCiV-1 和 PCrV-1 是在中国海南兴隆热带植物园流行的病毒，发生率超过 73%。
