Laboratory of Virology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém 66075-110, Brazil.
Graduate Program in Biology of Infectious and Parasitic Agents, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém 66075-110, Brazil.
Viruses. 2022 May 28;14(6):1175. doi: 10.3390/v14061175.
Genetic variations in components of the immune response seem to be an important factor that contributes to the manifestation of symptoms of some diseases related to HTLV-1 infection. Nerve growth factor (NGF) and the p75 neurotrophin receptor (p75NTR) are related to the maintenance of neurons and the activation of the immune response. In this study, we evaluated the association of the NGF -198C/T, NGF Ala35Val, and p75NTR Ser205Leu polymorphisms with HTLV-1 infection and plasma cytokine levels in 166 samples from individuals infected with HTLV-1 (59 symptomatic and 107 asymptomatic). The genotyping and quantification of the proviral load were performed by real-time PCR, and cytokine levels were measured by ELISA. The NGF -198C/T and NGF Ala35Val polymorphisms were not associated with HTLV-1 infection. The frequency of the Ser/Leu genotype of p75NTR Ser205Leu was more frequent in the control group (p = 0.0385), and the Ser/Leu genotype and allele Leu were more frequent among the asymptomatic (p < 0.05), especially with respect to the HTLV-1-associated myelopathy (HAM) group (p < 0.05). The symptomatic showed a higher proviral load and higher TNF-α and IL-10 levels (p < 0.05). Asymptomatic carriers of the Ser/Leu genotype (p = 0.0797) had lower levels of proviral load and higher levels of TNF-α (p = 0.0507). Based on the results obtained, we conclude that the p75NTR Ser205Leu polymorphism may be associated with reduced susceptibility to HTLV-1 infection, a lower risk of developing symptoms, including HAM, and better infection control.
免疫反应成分中的遗传变异似乎是导致某些与 HTLV-1 感染相关疾病症状表现的重要因素。神经生长因子（NGF）和 p75 神经营养素受体（p75NTR）与神经元的维持和免疫反应的激活有关。在这项研究中，我们评估了 NGF-198C/T、NGF Ala35Val 和 p75NTR Ser205Leu 多态性与 HTLV-1 感染和 166 例 HTLV-1 感染个体（59 例有症状和 107 例无症状）血浆细胞因子水平的相关性。通过实时 PCR 进行基因分型和前病毒载量定量，通过 ELISA 测量细胞因子水平。NGF-198C/T 和 NGF Ala35Val 多态性与 HTLV-1 感染无关。p75NTR Ser205Leu 的 Ser/Leu 基因型的频率在对照组中更为常见（p=0.0385），无症状组的 Ser/Leu 基因型和 Leu 等位基因更为常见（p<0.05），尤其是与 HTLV-1 相关的脊髓病（HAM）组（p<0.05）。有症状组的前病毒载量更高，TNF-α 和 IL-10 水平更高（p<0.05）。无症状携带者的 Ser/Leu 基因型（p=0.0797）具有较低的前病毒载量和较高的 TNF-α水平（p=0.0507）。基于获得的结果，我们得出结论，p75NTR Ser205Leu 多态性可能与降低 HTLV-1 感染易感性、降低发生包括 HAM 在内的症状的风险以及更好地控制感染有关。
