Laboratory of Virology, Institute of Biological Sciences, Federal University of Pará, Belém, Brazil.
Laboratory of Cellular and Molecular Biology, Tropical Medicine Center, Federal University of Pará, Belém, Brazil.
Front Cell Infect Microbiol. 2020 May 25;10:246. doi: 10.3389/fcimb.2020.00246. eCollection 2020.
SAMHD1, a host dNTPase, acts as a retroviral restriction factor by degrading the pool of nucleotides available for the initial reverse transcription of retroviruses, including HTLV-1. Polymorphisms in the gene may alter the enzymatic expression and influence the course of infection by the virus. The present study investigated the effect of polymorphisms on HTLV-1 infection susceptibility and on progression to disease in 108 individuals infected by HTLV-1 (47 symptomatic and 61 asymptomatic) and 100 individuals in a control group. rs6029941 (G/A) genotyping and HTLV-1 proviral load measurements were performed using real-time PCR and plasma IFN-α was measured by ELISA. Polymorphism frequency was not associated with HTLV-1 infection susceptibility or with the presence of symptoms. The proviral load was significantly higher in symptomatic individuals with the G allele ( = 0.0143), which presented lower levels of IFN-α ( = 0.0383). polymorphism is associated with increased proviral load and reduced levels of IFN-α in symptomatic patients, and may be a factor that contributes to the appearance of disease symptoms.
SAMHD1 是一种宿主 dNTP 酶，可通过降解用于逆转录病毒初始逆转录的核苷酸池来充当逆转录病毒的限制因子，包括 HTLV-1。基因中的多态性可能会改变酶的表达，并影响病毒的感染过程。本研究调查了多态性对 HTLV-1 感染易感性和对 108 名 HTLV-1 感染个体（47 名有症状和 61 名无症状）和 100 名对照组个体疾病进展的影响。使用实时 PCR 进行 rs6029941（G/A）基因分型和 HTLV-1 前病毒载量测量，并通过 ELISA 测量血浆 IFN-α。多态性频率与 HTLV-1 感染易感性或症状存在无关。具有 G 等位基因的有症状个体的前病毒载量显着更高（= 0.0143），其 IFN-α水平较低（= 0.0383）。多态性与有症状患者的前病毒载量增加和 IFN-α水平降低有关，可能是导致疾病症状出现的一个因素。
