Yang Jun, Li Xiaoqian, Yang Xuefeng, Wei Hongjiang, Deng Lipu, Fu Nian
Department of Emergency, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan 421000, P.R. China.
Department of Gastroenterology, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan 421000, P.R. China.
Exp Ther Med. 2022 May 20;24(1):458. doi: 10.3892/etm.2022.11385. eCollection 2022 Jul.
Cell division cycle 42 (CDC42) can inhibit inflammation by regulating the activity of macrophage and T cells, which contributes to the pathophysiology of acute pancreatitis (AP). Therefore, CDC42 may have application as a potential biomarker for AP. The present study aimed to explore this possibility. Peripheral blood mononuclear cells (PBMCs) were collected from 149 patients with AP and 50 healthy controls (HCs). Subsequently, CDC42 expression in the PBMCs was measured using RT-qPCR; C-reactive protein (CRP), TNF-α and IL-6 in the serum of patients with AP were measured using ELISA. Meanwhile, Mann-Whitney U test, Kruskal-Wallis test, and Spearman's rank correlation test were performed on the data. The CDC42 expression levels were lower in patients with AP compared with those in HCs (P<0.001). CDC42 expression was declined in patients with moderate-severe AP (MSAP) vs. patients with mild AP (MAP) (P=0.029), and in patients with severe AP (SAP) vs. patients with MAP (P=0.004). CDC42 expression correlated negatively with the Ranson's score (P<0.001), APACEH II score (P=0.011) and SOFA score (P<0.001) in patients with AP. CDC42 expression also correlated negatively with CRP (P<0.001) and TNF-α (P=0.004) levels but not with IL-6 levels (P=0.177). Furthermore, CDC42 expression was lower in deceased patients with AP vs. AP survivors (P<0.001) and in deceased patients with SAP vs. SAP survivors (P=0.026). CDC42 had good potential in predicting mortality from AP, with AUC of 0.829 and a 95% CI of 0.731-0.927, and it also had certain potential in predicting mortality from SAP and MSAP, with AUC (95% CI) of 0.794 (0.616-0.973) and 0.757 (0.558-0.956), respectively. In conclusion, data from the present study suggest that lower CDC42 expression levels correlate with higher disease susceptibility, disease severity, inflammation, and mortality risk in patients with AP.
细胞分裂周期42（CDC42）可通过调节巨噬细胞和T细胞的活性来抑制炎症，这与急性胰腺炎（AP）的病理生理学有关。因此，CDC42可能作为AP的潜在生物标志物。本研究旨在探讨这种可能性。收集了149例AP患者和50名健康对照者（HCs）的外周血单个核细胞（PBMCs）。随后，采用逆转录定量聚合酶链反应（RT-qPCR）检测PBMCs中CDC42的表达；采用酶联免疫吸附测定（ELISA）检测AP患者血清中的C反应蛋白（CRP）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）。同时，对数据进行曼-惠特尼U检验、克鲁斯卡尔-沃利斯检验和斯皮尔曼等级相关检验。与HCs相比，AP患者的CDC42表达水平较低（P<0.001）。中度-重度AP（MSAP）患者的CDC42表达低于轻度AP（MAP）患者（P=0.029），重度AP（SAP）患者的CDC42表达低于MAP患者（P=0.004）。AP患者的CDC42表达与兰森评分（P<0.001）、急性生理与慢性健康状况评分系统II（APACHE II）评分（P=0.011）和序贯器官衰竭评估（SOFA）评分（P<0.001）呈负相关。CDC42表达也与CRP（P<0.001）和TNF-α水平（P=0.004）呈负相关，但与IL-6水平无关（P=0.177）。此外，AP死亡患者的CDC42表达低于AP存活患者（P<0.001），SAP死亡患者的CDC42表达低于SAP存活患者（P=0.026）。CDC42在预测AP死亡率方面具有良好的潜力，曲线下面积（AUC）为0.829，95%置信区间（CI）为0.731-0.927，在预测SAP和MSAP死亡率方面也具有一定潜力，AUC（95%CI）分别为0.794（0.616-0.973）和0.757（0.558-0.956）。总之,本研究数据表明，较低的CDC42表达水平与AP患者较高的疾病易感性、疾病严重程度、炎症和死亡风险相关。
