Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia.
Department of Histology and Embryology, University of Zagreb School of Medicine, Zagreb, Croatia.
J Cereb Blood Flow Metab. 2022 Nov;42(11):2080-2094. doi: 10.1177/0271678X221109873. Epub 2022 Jun 24.
Cerebral and retinal ischemia share similar pathogenesis and epidemiology, each carrying both acute and prolonged risk of the other and often co-occurring. The most used preclinical stroke models, the Koizumi and Longa middle cerebral artery occlusion (MCAO) methods, have reported retinal damage with great variability, leaving the disruption of retinal blood supply via MCAO poorly investigated, even providing conflicting assumptions on the origin of the ophthalmic artery in rodents. The aim of our study was to use longitudinal magnetic resonance assessment of cerebral and retinal vascular perfusion after the ischemic injury to clarify whether and how the Koizumi and Longa methods induce retinal ischemia and how they differ in terms of cerebral and retinal lesion evolution. We provided anatomical evidence of the origin of the ophthalmic artery in mice from the pterygopalatine artery. Following the Koizumi surgery, retinal responses to ischemia overlapped with those in the brain, resulting in permanent damage. In contrast, the Longa method produced only extensive cerebral lesions, with greater tissue loss than in the Koizumi method. Additionally, our data suggests the Koizumi method should be redefined as a model of ischemia with chronic hypoperfusion rather than of ischemia and reperfusion.
脑和视网膜缺血具有相似的发病机制和流行病学特征，两者都存在急性和长期的风险，并且经常同时发生。最常用的临床前中风模型，如 Koizumi 和 Longa 大脑中动脉闭塞 (MCAO) 方法，已经报道了视网膜损伤的巨大变异性，使得 MCAO 导致视网膜血液供应中断的研究不足，甚至对啮齿动物眼动脉的起源提供了相互矛盾的假设。我们的研究旨在通过缺血损伤后对脑和视网膜血管灌注的纵向磁共振评估，阐明 Koizumi 和 Longa 方法是否以及如何引起视网膜缺血，以及它们在脑和视网膜病变演变方面有何不同。我们提供了来自翼腭动脉的小鼠眼动脉起源的解剖学证据。在 Koizumi 手术后，视网膜对缺血的反应与大脑的反应重叠，导致永久性损伤。相比之下，Longa 方法仅产生广泛的大脑病变，其组织损失比 Koizumi 方法更大。此外，我们的数据表明，Koizumi 方法应重新定义为慢性低灌注而非缺血再灌注的模型。
