评估新诊断的沙特类风湿关节炎患者的白细胞介素-1β、白细胞介素-6、肿瘤坏死因子-α、白细胞介素-8 和 C 趋化因子配体 5 水平。

Assessment of IL-1β, IL-6, TNF-α, IL-8, and CCL 5 levels in newly diagnosed Saudi patients with rheumatoid arthritis.

机构信息

Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Majmaah, Saudi Arabia.

Main Laboratory and blood bank, Alzulfi General Hospital, Alzulfi, Saudi Arabia.

出版信息

Int J Rheum Dis. 2022 Sep;25(9):1013-1019. doi: 10.1111/1756-185X.14373. Epub 2022 Jun 24.

DOI:10.1111/1756-185X.14373

PMID:35748059

Abstract

BACKGROUND

Rheumatoid arthritis (RA) is a chronic systemic inflammatory disorder which mainly affects small joints, occurs most commonly in middle-aged adults, and can be fatal in severe cases. The exact etiology of RA remains unknown. However, uncontrolled expression of pro-inflammatory cytokines and chemokines can contribute to the pathogenesis of RA.

AIM

In the current study, we assessed the potential of serum concentrations of interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, IL-8, and C-C motif chemokine ligand (CCL)5 as early predictive markers for RA.

METHODS

In addition to clinical examination, blood samples were collected from 100 Saudi patients recently diagnosed with early RA for basic and serological tests, including rheumatoid factor (RF), C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR). Sera of 32 healthy individuals were used as controls. Specific enzyme-linked immunosorbent assay was used to quantify the serum IL-1β, IL-6, TNF-α, IL-8, and CCL5 levels in the samples.

RESULTS

Our results indicated that RF, CRP, and ESR levels were higher in RA patients compared to controls. Furthermore, serum levels of IL-1β, IL-6, IL-8, and CCL5, but not TNF-α, significantly increased in RA patients compared to controls.

CONCLUSION

Overall, the findings suggested that IL-1β, IL-6, IL-8, and CCL5 can be used as biomarkers in the early diagnosis of RA.

摘要

背景

类风湿关节炎（RA）是一种慢性系统性炎症性疾病，主要影响小关节，最常见于中年成年人，在严重情况下可能是致命的。RA的确切病因尚不清楚。然而，促炎细胞因子和趋化因子的失控表达可能导致 RA 的发病机制。

目的

在本研究中，我们评估了血清白细胞介素（IL）-1β、IL-6、肿瘤坏死因子（TNF）-α、IL-8 和 C-C 基序趋化因子配体（CCL）5 浓度作为 RA 早期预测标志物的潜力。

方法

除临床检查外，还从 100 名最近被诊断为早期 RA 的沙特患者采集血液样本进行基础和血清学检查，包括类风湿因子（RF）、C 反应蛋白（CRP）和红细胞沉降率（ESR）。将 32 名健康个体的血清作为对照。使用特定的酶联免疫吸附试验来定量样品中的血清 IL-1β、IL-6、TNF-α、IL-8 和 CCL5 水平。