口腔肿瘤学中的纳米孔测序技术：全面洞察。

Nanopore Sequencing Technology in Oral Oncology: A Comprehensive Insight.

机构信息

Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, MS Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India.

College of Dentistry, Jouf University, Sakaka, Saudi Arabia.

出版信息

J Contemp Dent Pract. 2022 Feb 1;23(2):268-275.

PMID:35748459

Abstract

AIM

To review the principles and application of Nanopore Sequencing Technology (NPST) in oral cancer.

BACKGROUND

Oral cancer is a disease caused by aberrations in the genes. Substantial research at the genomic level is still required for in-depth understanding of the molecular mechanism in oral cancer. The advent of the novel nanopore sequencing technique has the potential to detect the alterations at the genomic level. This review highlights nanopore sequencing, its advantages and disadvantages, and how research supports its application in the field of oral oncology.

MATERIALS AND METHODS

Web-based search via PubMed database, internet sources using keywords "nanopore sequencing, third-generation sequencing, next generation sequencing, cancer, oral squamous cell carcinoma, genetic, epigenetic, oncogenic viruses" was performed in this review. Original research, reviews, and short discussions published from 2008 to 2020 were included. The findings are discussed with emphasis on common gene mutations, epigenetic alterations, and oncogenic viruses in oral cancer. A brief mention regarding translational nanopore sequencing research in oral cancer and future perspectives is also discussed.

RESULTS

The results obtained reveal that cost-effectiveness and rapid turnaround time make nanopore sequencing an enticing platform to resolve the ambiguity of genomes, epigenomes, and transcriptomes.

CONCLUSION

The findings will encourage researchers to further adopt NPST in their studies and give an overview of the latest findings of oral squamous cell carcinoma (OSCC) management. To highlight the importance of NPST application in OSCC studies, this paper not only discusses the use of NPST in identifying the behavior of malignancy but also implies the need for further research using this technique.

CLINICAL SIGNIFICANCE

The review suggests that nanopore sequencing can be utilized for diagnosis and achieving personalized treatment in each oral cancer patient.

摘要

目的

综述纳米孔测序技术（Nanopore Sequencing Technology，NPST）在口腔癌中的原理和应用。

背景

口腔癌是一种由基因异常引起的疾病。为了深入了解口腔癌的分子机制，仍需要在基因组水平上进行大量研究。新型纳米孔测序技术的出现有可能检测到基因组水平的改变。本综述强调了纳米孔测序技术及其优缺点，以及研究如何支持其在口腔肿瘤学领域的应用。

材料和方法

通过 PubMed 数据库进行网络搜索，利用关键词“纳米孔测序、第三代测序、下一代测序、癌症、口腔鳞状细胞癌、遗传、表观遗传、致癌病毒”在互联网上搜索，纳入了 2008 年至 2020 年发表的原始研究、综述和短篇讨论。讨论结果重点关注口腔癌中常见的基因突变、表观遗传改变和致癌病毒。还简要提到了纳米孔测序在口腔癌中的转化研究和未来展望。