Department of Hepatobiliary Surgery, the First Affiliated Hospital of Hunan Normal University (Hunan Provincial People's Hospital), Changsha, Hunan, People's Republic of China.
Kaohsiung J Med Sci. 2022 Sep;38(9):826-838. doi: 10.1002/kjm2.12566. Epub 2022 Jun 24.
To investigate the effect of long noncoding RNA (LINC01419)/miR-485-5p/LSM4 on the malignant behavior of hepatocellular carcinoma (HCC) cells. The expressions of LINC01419, miR-485-5p, and LSM4 were determined in HCC at the cellular and clinical levels, and cell biological behavior was evaluated. The relationships between LINC01419, miR-485-5p, and LSM4 were predicted and verified. Additionally, the subcellular localization of LINC01419 in HCC cells was analyzed. Finally, an animal experiment was conducted to confirm the effect of LINC01419 silencing on tumor growth. in HCC tissues and cells, LINC01419 and LSM4 were increasingly expressed, but miR-485-5p was decreasingly expressed. LINC01419 negatively regulated miR-485-5p- and miR-485-5p-targeted LSM4. LINC01419 was localized in the cytoplasm of HCC cells. Downregulation of miR-485-5p or upregulation of LSM4 reversed the inhibition of HCC cell malignant behavior by LINC01419 interference. LINC01419 sponges miR-485-5p to upregulate LSM4 expression, thereby facilitating the biological behavior of HCC cells.
为了探究长链非编码 RNA（LINC01419）/miR-485-5p/LSM4 对肝癌（HCC）细胞恶性行为的影响。在细胞和临床水平上测定了 HCC 中 LINC01419、miR-485-5p 和 LSM4 的表达，并评估了细胞生物学行为。预测并验证了 LINC01419、miR-485-5p 和 LSM4 之间的关系。此外，分析了 LINC01419 在 HCC 细胞中的亚细胞定位。最后，进行了动物实验以证实 LINC01419 沉默对肿瘤生长的影响。在 HCC 组织和细胞中，LINC01419 和 LSM4 的表达增加，而 miR-485-5p 的表达减少。LINC01419 负调控 miR-485-5p-和 miR-485-5p 靶向的 LSM4。LINC01419 定位于 HCC 细胞的细胞质中。下调 miR-485-5p 或上调 LSM4 逆转了 LINC01419 干扰对 HCC 细胞恶性行为的抑制作用。LINC01419 作为 miR-485-5p 的海绵，上调 LSM4 的表达，从而促进 HCC 细胞的生物学行为。
