Fernandes Diégina Araújo, Rique Hyago Luiz, de Oliveira Louise Helena Guimarães, Santos Wilias Greison Silva, de Souza Maria de Fátima Vanderlei, Nunes Fabiola da Cruz
Pharmacist, MSc. Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos (PgPNSB), Universidade Federal da Paraíba (UFPB), João Pessoa, PB, Brasil.
Curso de Graduação em Biotecnologia, UFPB, João Pessoa, PB, Brasil.
Braz J Vet Med. 2021 Mar 26;43:e112120. doi: 10.29374/2527-2179.bjvm112120. eCollection 2021.
is a vector of emerging and neglected diseases, such as dengue, chikungunya, and Zika. known as "pitó" in Brazil, is traditionally used as an insect repellent, and several studies have demonstrated its larvicidal activity. The aim of this study was to investigate this species and evaluate its potential ovicidal, pupicidal, adulticidal, and repellent activity. The viability of the eggs was evaluated using different concentrations of the test substances for 25 days. The hexane fraction killed 72.7% of the eggs, while dichloromethane killed 67.7%. The survival of the pupae and adults was verified after 72 h and 48 h, respectively. The LC for the hexane and dichloromethane fractions was 0.12 mg/mL and 8.85 mg/mL for pupae, 8.01 mg/mL and 0.74 mg/mL for adults (tarsal test), and 0.05 mg/mL and 0.23 mg/mL for adults (body test), respectively. Repellency was assessed for 240 min using neonatal Wistar rats on a Y-tube olfactometer. The hexane fraction attracted mosquitoes to the test chamber, while the dichloromethane fraction had a repellent action. The 7,4'-di--methyl-8--sulfate flavone provides greater repellency, and this finding is similar to the results of the studies that have shown the potential of this substance against adult mosquitoes. This suggests that 7,4'-di--methyl-8--sulfate flavone may be one of the substances present in the extract from aerial parts of that is responsible for the repellent activity mentioned in traditional medicine. These findings provide a better understanding of the insecticidal and repellent activity of the extract, fraction, and compounds isolated from against thereby revealing its potential in the development of a more effective botanical insecticide.
是登革热、基孔肯雅热和寨卡等新出现的被忽视疾病的传播媒介。在巴西被称为“pitó”，传统上用作驱虫剂，多项研究已证明其杀幼虫活性。本研究的目的是调查该物种并评估其潜在的杀卵、杀蛹、杀成虫和驱避活性。使用不同浓度的测试物质评估卵的活力，持续25天。己烷馏分杀死了72.7%的卵，而二氯甲烷杀死了67.7%。分别在72小时和48小时后验证蛹和成虫的存活率。己烷和二氯甲烷馏分对蛹的半数致死浓度分别为0.12毫克/毫升和8.85毫克/毫升，对成虫（跗节测试）分别为8.01毫克/毫升和0.74毫克/毫升，对成虫（身体测试）分别为0.05毫克/毫升和0.23毫克/毫升。使用新生Wistar大鼠在Y型嗅觉仪上评估驱避性240分钟。己烷馏分将蚊子吸引到测试室，而二氯甲烷馏分具有驱避作用。7,4'-二甲基-8-硫酸黄酮具有更强的驱避性，这一发现与已表明该物质对成年蚊子有潜在作用的研究结果相似。这表明7,4'-二甲基-8-硫酸黄酮可能是该植物地上部分提取物中具有传统医学中提到的驱避活性的物质之一。这些发现有助于更好地理解从该植物中分离出的提取物、馏分和化合物对蚊子的杀虫和驱避活性，从而揭示其在开发更有效的植物杀虫剂方面的潜力。
