Fouda Amr, Eid Ahmed M, Abdel-Rahman Mohamed Ali, El-Belely Ehab F, Awad Mohamed A, Hassan Saad El-Din, Al-Faifi Zarraq E, Hamza Mohammed F
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.
Department of Zoology and Entomology, Faculty of Science, Al-Azhar University, Cairo, Egypt.
Front Bioeng Biotechnol. 2022 Feb 28;10:849921. doi: 10.3389/fbioe.2022.849921. eCollection 2022.
Herein, the metabolites secreted by brown algae, , were used as biocatalyst for green synthesis of magnesium oxide nanoparticles (MgO-NPs). The fabricated MgO-NPs were characterized using UV-vis spectroscopy, Fourier transforms infrared spectroscopy (FT-IR), Transmission Electron Microscopy (TEM), Scanning Electron Microscopy linked with energy-dispersive X-ray (SEM-EDX), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). Data showed successful formation of crystallographic and spherical MgO-NPs with sizes of 3-18 nm at a maximum surface plasmon resonance of 320 nm. Moreover, EDX analysis confirms the presence of Mg and O in the sample with weight percentages of 54.1% and 20.6%, respectively. Phyco-fabricated MgO-NPs showed promising activities against Gram-positive bacteria, Gram-negative bacteria, and with MIC values ranging between 12.5 and 50 μg mL. The IC value of MgO-NPs against cancer cell lines (Caco-2) was 113.4 μg mL, whereas it was 141.2 μg mL for normal cell lines (Vero cell). Interestingly, the green synthesized MgO-NPs exhibited significant larvicidal and pupicidal activity against At 10 μg mL MgO-NPs, the highest mortality percentages were 99.0%, 95.0%, 92.2%, and 81.0% for I, II, III instars' larvae, and pupa of , respectively, with LC values (3.08, 3.49, and 4.46 μg mL), and LC values (7.46, 8.89, and 10.43 μg mL), respectively. Also, MgO-NPs showed repellence activity for adults of at 10 μg mL with 63.0%, 77.9%, 84.9%, and 96.8% after 12, 24, 48, and 72 h, respectively.
在此，褐藻分泌的代谢产物被用作绿色合成氧化镁纳米颗粒（MgO-NPs）的生物催化剂。使用紫外可见光谱、傅里叶变换红外光谱（FT-IR）、透射电子显微镜（TEM）、与能量色散X射线联用的扫描电子显微镜（SEM-EDX）、X射线衍射（XRD）和X射线光电子能谱（XPS）对制备的MgO-NPs进行了表征。数据显示成功形成了晶体状和球形的MgO-NPs，尺寸为3 - 18纳米，最大表面等离子体共振为320纳米。此外，EDX分析证实样品中存在Mg和O，重量百分比分别为54.1%和20.6%。藻类制备的MgO-NPs对革兰氏阳性菌、革兰氏阴性菌显示出有前景的活性，对[此处原文缺失一种菌名]的最小抑菌浓度（MIC）值在12.5至50微克/毫升之间。MgO-NPs对癌细胞系（Caco-2）的半数抑制浓度（IC）值为113.4微克/毫升，而对正常细胞系（Vero细胞）为141.2微克/毫升。有趣的是，绿色合成的MgO-NPs对[此处原文缺失一种虫名]表现出显著的杀幼虫和杀蛹活性。在10微克/毫升的MgO-NPs浓度下，对[此处原文缺失一种虫名]的一龄、二龄、三龄幼虫和蛹的最高死亡率分别为99.0%、95.0%、92.2%和81.0%，其半数致死浓度（LC）值分别为（3.08、3.49和4.46微克/毫升）以及（7.46、8.89和10.43微克/毫升）。此外，MgO-NPs在10微克/毫升时对[此处原文缺失一种虫名]成虫显示出驱避活性，在12、24、48和72小时后的驱避率分别为63.0%、77.9%、84.9%和96.8%。
