Kowanko I C, Ferrante A, Maxwell G M
Pediatr Res. 1987 Apr;21(4):377-80. doi: 10.1203/00006450-198704000-00011.
Neonatal neutrophil migration was inhibited by preincubation with a lymphokine/monokine-rich medium conditioned by phytohemagglutinin-stimulated mononuclear leucocytes. Medium conditioned by unstimulated mononuclear cells or nonconditioned medium had no effect on neonatal neutrophil migration. Similar results were obtained with adult neutrophils. Migration distances in the presence and absence of a chemotactic gradient were much lower for neutrophils from neonates than adults when comparing treatments with the corresponding medium, i.e. medium conditioned by phytohemagglutinin-stimulated mononuclear leucocytes, medium conditioned by unstimulated mononuclear leucocytes, or medium unconditioned by mononuclear leucocytes (p less than 0.01). Although locomotion of both neonatal and adult neutrophils was inhibited by treatment, the percent inhibition of random migration was slightly but significantly less for neonates than adults (p less than 0.05). These results demonstrate that neutrophils from neonates are modulated by mononuclear leucocyte-derived mediators.
用植物血凝素刺激的单核白细胞条件培养液（富含淋巴因子/单核因子）预孵育可抑制新生儿中性粒细胞的迁移。未刺激的单核细胞条件培养液或未条件培养液对新生儿中性粒细胞迁移没有影响。成人中性粒细胞也得到了类似结果。当用相应培养液（即植物血凝素刺激的单核白细胞条件培养液、未刺激的单核白细胞条件培养液或未用单核白细胞条件培养液）处理时，与成人相比，新生儿中性粒细胞在有和没有趋化梯度情况下的迁移距离要低得多（p<0.01）。虽然处理均抑制了新生儿和成人中性粒细胞的运动，但新生儿随机迁移的抑制百分比略低于成人且差异有统计学意义（p<0.05）。这些结果表明，新生儿中性粒细胞受到单核白细胞衍生介质的调节。
