State Key Laboratory for Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
State Key Laboratory for Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Nov 5;280:121522. doi: 10.1016/j.saa.2022.121522. Epub 2022 Jun 17.
A highly sensitive smartphone-integrated fluorescence quenching immunochromatographic assay (FQICA) for the detection of sesame allergen was proposed. Sesame antibodies were adsorbed on the surface of the gold nanoparticles to form fluorescent acceptors (AuNPs-Ab). Ovalbumin (OVA) protein was labeled with quantum dots (QDs) to form signal probes (QDs-OVA), which were coated on the C-line of the assay strips. A mixture of QDs-OVA and sesame protein was coated on the T-line of the strip. For FQICA, the concentration of the analyte was positively correlated with the fluorescence signal. The developed FQICA had high sensitivity for the detection of sesame protein, and its visual LOD was 80 μg/L and the quantitative LOD was 40 μg/L. In addition, the method had high specificity, except for a small cross-reaction between sesame and walnut. The visual LODs in bread, ham, and biscuits were 640 μg/L. The quantitative LODs were 320 μg/L for biscuits and 640 μg/L for bread and ham. Comparing the developed FQICA with a commercial ELISA kit, the recoveries of sesame protein in both methods were between 80% and 120%.
提出了一种用于检测芝麻过敏原的高灵敏度智能手机集成荧光猝灭免疫层析分析（FQICA）方法。芝麻抗体被吸附在金纳米粒子的表面上形成荧光受体（AuNPs-Ab）。卵清蛋白（OVA）蛋白被标记上量子点（QDs）形成信号探针（QDs-OVA），并涂覆在检测带的 C 线上。QDs-OVA 和芝麻蛋白的混合物涂覆在条带的 T 线上。对于 FQICA，分析物的浓度与荧光信号呈正相关。开发的 FQICA 对芝麻蛋白的检测具有很高的灵敏度，其目视 LOD 为 80μg/L，定量 LOD 为 40μg/L。此外，该方法具有很高的特异性，除了芝麻和核桃之间存在较小的交叉反应。在面包、火腿和饼干中，目视 LOD 分别为 640μg/L、320μg/L 和 640μg/L。与商业 ELISA 试剂盒相比，两种方法中芝麻蛋白的回收率均在 80%到 120%之间。
