Department of Physical and Analytical Chemistry, University of Oviedo, c/Julián Clavería, 8, 33006 Oviedo, Spain.
International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga Sthis n, 4715-330 Braga, Portugal.
Biosensors (Basel). 2024 Aug 3;14(8):377. doi: 10.3390/bios14080377.
Food safety control is a key issue in the food and agriculture industries. For such purposes, developing miniaturized analytical methods is critical for enabling the rapid and sensitive detection of food supplements, allergens, and pollutants. Here, a novel bioanalytical methodology based on DNA-functionalized gold nanoparticles (AuNPs) and colorimetric detection was developed to detect the presence of sesame (a major allergen) through sesame seed DNA as a target, in food samples. The presence of sesame DNA induces controlled nanoparticle aggregation/desegregation, resulting in a color change (from blue to red) proportional to sesame DNA concentration. The incorporation of multicomponent nucleic acid enzymes (MNAzymes) in this strategy has been carried out to perform an isothermal signal amplification strategy to improve the sensitivity of detection. Also, open-source software for color analysis was used to ensure an unbiased visual color-change detection, enhancing detection accuracy and sensitivity and opening the possibility of performing a simple and decentralized analyte detection. The method successfully detected the presence of sesame DNA in sesame seed, sesame oil, olive oil, and sunflower oil. In brief, the developed approach constitutes a simple and affordable alternative to perform a highly sensitive detection of DNA in food without complex methodologies or the requirement of expensive instrumentation.
食品安全控制是食品和农业产业的关键问题。为此，开发小型化分析方法对于实现食品补充剂、过敏原和污染物的快速灵敏检测至关重要。在这里，开发了一种基于 DNA 功能化金纳米粒子 (AuNPs) 和比色检测的新型生物分析方法，通过芝麻种子 DNA 作为靶标，用于检测食品样品中芝麻（一种主要过敏原）的存在。芝麻 DNA 的存在诱导了受控的纳米粒子聚集/解聚集，导致颜色变化（从蓝色变为红色）与芝麻 DNA 浓度成正比。在该策略中引入了多组分核酸酶 (MNAzymes)，以进行等温信号放大策略，从而提高检测的灵敏度。此外，还使用了用于颜色分析的开源软件，以确保进行无偏的视觉颜色变化检测，从而提高检测的准确性和灵敏度，并为进行简单和分散的分析物检测开辟了可能性。该方法成功地检测到了芝麻种子、芝麻油、橄榄油和葵花籽油中芝麻 DNA 的存在。总之，所开发的方法构成了一种简单且经济实惠的替代方法，可在无需复杂方法或昂贵仪器的情况下，对食品中的 DNA 进行高度灵敏的检测。
