Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, Liaoning, 116023, PR China; College of Life Science, Liaoning Normal University, Dalian, Liaoning, 116029, PR China.
Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, Liaoning, 116023, PR China.
Microb Pathog. 2022 Aug;169:105651. doi: 10.1016/j.micpath.2022.105651. Epub 2022 Jun 22.
The sea urchin Strongylocentrotus intermedius, famous for its gonadal quality, is one of the most important farmed species in the sea area of northern China. Since 2020, outbreaks of black peristomial membrane disease (commonly called black mouth disease) have frequently occurred in spring and winter in cultured S. intermedius. In this study, we isolated the predominant bacteria from different tissues of diseased sea urchins from a North China farm in the spring of 2021. Four pathogenic strains (named SIBMPM01, SIBMPM02, SIBMPM03 and SIBMCF01) were obtained and characterized by Gram staining, morphological observation, artificial infection tests, and metabolic characteristics. Our results showed that: 1) all obtained strains belonged to the genus Vibrio and had morphological differences. Phylogenetic analysis indicated that the four obtained strains might be novel Vibrio species. 2) Laboratory-based artificial infection tests showed that sea urchins infected with either SIBMPM01, SIBMPM02, SIBMPM03 or SIBMCF01 exhibited pathological symptoms of a black peristomial membrane in a dosage-dependent and temperature-dependent manner. The virulence of SIBMCF01 was greater than those of the others. 3) Metabolic characterization data showed that SIBMPM01, SIBMPM02, SIBMPM03 and SIBMCF01 shared similar metabolic characteristics. 4) Antimicrobial susceptibility tests demonstrated that the four obtained strains were all sensitive to ampicillin, doxycycline, norfloxacin, ofloxacin, furazolidone and chloramphenicol. SIBMPM01 was specifically sensitive to neomycin, and SIBMCF01 was specifically sensitive to carboxybenzyl penicillin.
棘皮动物海胆是一种以生殖腺质量而闻名的物种，是中国北方海域最重要的养殖物种之一。自 2020 年以来，养殖中间球海胆（Strongylocentrotus intermedius）在春冬季频繁暴发黑口病。本研究于 2021 年春季从中国北方某养殖场采集患病海胆的不同组织，分离获得优势病原菌，并进行了革兰氏染色、形态观察、人工感染实验和代谢特性等分析。结果表明：1）从患病海胆中分离得到的 4 株病原菌（命名为 SIBMPM01、SIBMPM02、SIBMPM03 和 SIBMCF01）均属于弧菌属（Vibrio），且形态存在差异；系统发育分析显示，这 4 株菌可能为弧菌属新种。2）实验室人工感染实验结果显示，海胆经 SIBMPM01、SIBMPM02、SIBMPM03 或 SIBMCF01 感染后，均表现出黑口病的典型病理症状，且具有剂量和温度依赖性，其中 SIBMCF01 的致病力最强。3）代谢特性分析显示，SIBMPM01、SIBMPM02、SIBMPM03 和 SIBMCF01 具有相似的代谢特性。4）药敏实验结果显示，4 株菌对氨苄西林、强力霉素、诺氟沙星、氧氟沙星、呋喃唑酮和氯霉素均敏感，SIBMPM01 对新霉素敏感，SIBMCF01 对羧苄西林敏感。
