College of Coastal Agricultural Sciences, School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China.
College of Coastal Agricultural Sciences, School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China; Shenzhen Research Institute of Guangdong Ocean University, Shenzhen 518108, China.
Sci Total Environ. 2022 Oct 15;843:156817. doi: 10.1016/j.scitotenv.2022.156817. Epub 2022 Jun 21.
Soil salinization has been recognized as one of the main factors causing the decrease of cultivated land area and global plant productivity. Application of salt tolerant plants and improvement of plant salt tolerance are recognized as the major routes for saline soil restoration and utilization. Sea rice 86 (SR86) is known as a rice cultivar capable of growing in saline soil. Genome sequencing and transcriptome analysis of SR86 have been conducted to explore its salt tolerance mechanisms while the contribution of rhizobacteria is underexplored. In the present study, we examined the rhizosphere bacterial diversity and soil metabolome of SR86 seedlings under different salinity to understand their contribution to plant salt tolerance. We found that salt stress could significantly change rhizobacterial diversity and rhizosphere metabolites. Keystone taxa were identified via co-occurrence analysis and the correlation analysis between keystone taxa and rhizosphere metabolites indicated lipids and their derivatives might play an important role in plant salt tolerance. Further, four plant growth promoting rhizobacteria (PGPR), capable of promoting the salt tolerance of SR86, were isolated and characterized. These findings might provide novel insights into the mechanisms of plant salt tolerance mediated by plant-microbe interaction, and promote the isolation and application of PGPR in the restoration and utilization of saline soil.
土壤盐渍化已被认为是导致耕地面积减少和全球植物生产力下降的主要因素之一。应用耐盐植物和提高植物耐盐性被认为是盐碱地修复和利用的主要途径。海水稻 86（SR86）被认为是一种能够在盐碱地生长的水稻品种。为了探索其耐盐机制，对 SR86 进行了基因组测序和转录组分析，但对根际细菌的贡献研究较少。在本研究中，我们研究了不同盐度下 SR86 幼苗的根际细菌多样性和土壤代谢组，以了解它们对植物耐盐性的贡献。结果表明，盐胁迫可以显著改变根际细菌多样性和根际代谢物。通过共现分析鉴定出关键类群，关键类群与根际代谢物的相关性分析表明，脂质及其衍生物可能在植物耐盐性中发挥重要作用。此外，还分离和鉴定了 4 株能够促进 SR86 耐盐性的植物促生根际细菌（PGPR）。这些发现可能为植物-微生物相互作用介导的植物耐盐机制提供新的见解，并促进 PGPR 在盐碱地修复和利用中的分离和应用。
