Lloyd P E, Kupfermann I, Weiss K R
Peptides. 1987 Jan-Feb;8(1):179-84. doi: 10.1016/0196-9781(87)90184-7.
Previous studies have shown that the nervous system and other tissues of molluscs contain a number of peptides that potently excite molluscan hearts. Two such peptides, termed small cardioactive peptides A and B (SCPA and SCPB) are present in large quantities in the nervous system of Aplysia. These peptides are widely distributed within the CNS and peripheral tissues and have been found to be potent modulators of synaptic transmission in Aplysia. SCPB has previously been purified from nervous tissue and sequenced. In this paper, we report the purification of SCPA and propose its sequence. This sequence was confirmed by comparing the chromatographic properties of native SCPA (labelled in organ culture) with a synthetic peptide that has the proposed sequence. A significant proportion of the sequence of the two SCPs is conserved, indicating that they are members of the same peptide class, a finding that is consistent with the recent observation that the two peptide sequences are present in a single precursor.
先前的研究表明，软体动物的神经系统和其他组织含有多种能有效刺激软体动物心脏的肽。两种这样的肽，称为小活性心脏肽A和B（SCPA和SCPB），大量存在于海兔的神经系统中。这些肽广泛分布于中枢神经系统和外周组织，并已被发现是海兔突触传递的有效调节剂。SCPB先前已从神经组织中纯化并测序。在本文中，我们报告了SCPA的纯化及其序列的提出。通过比较天然SCPA（在器官培养中标记）与具有所提出序列的合成肽的色谱特性，证实了该序列。这两种SCPs的很大一部分序列是保守的，表明它们是同一肽类的成员，这一发现与最近观察到的这两种肽序列存在于单个前体中的结果一致。
