对来自蜗牛（Helix aspersa）大脑的一种新型心脏抑制肽的部分特性分析。
Partial characterization of a novel cardioinhibitory peptide from the brain of the snail Helix aspersa.
作者信息
Baud C, Darbon P, Li K W, Marchand C R
机构信息
Laboratoire de Biologie des Organismes et Ecosystèmes, UFR Sciences et Techniques, Besançon, France.
出版信息
Cell Mol Neurobiol. 1998 Aug;18(4):413-24. doi: 10.1023/a:1022549515664.
Abstract
- We report the isolation of a peptide from the brain of the snail Helix aspersa by radioimmunoassay using an antisomatostatin. 2. The sequencing of an immunopositive fraction showed the presence of a new tridecapeptide, termed Helix cardioinhibitory peptide (HCIP), with the following primary structure: H-Val-Phe-Gln-Asn-Gln-Phe-Lys-Gly-Ile-Gln-Gly-Arg-Phe-NH2. It is structurally related to the Achatina cardioexcitatory peptide (ACEP-1) and the terminal-amino acid sequence of HCIP is identical to that of FMRFamide family peptides. 3. The synthetic HCIP was tested on heart and neuronal activities and it was found to have inhibitory actions not only on the ventricle but also on visceral neurons of the central nervous system of Helix. Immunocytochemical investigation indicates its presence in visceral and parietal ganglia, in which cells taking part in the regulation of the heartbeat have been previously identified.
摘要
- 我们通过使用抗生长抑素的放射免疫分析法，报告了从蜗牛（Helix aspersa）大脑中分离出一种肽。2. 对免疫阳性部分进行测序显示存在一种新的十三肽，称为螺旋心脏抑制肽（HCIP），其一级结构如下：H-缬氨酸-苯丙氨酸-谷氨酰胺-天冬酰胺-谷氨酰胺-苯丙氨酸-赖氨酸-甘氨酸-异亮氨酸-谷氨酰胺-甘氨酸-精氨酸-苯丙氨酸-NH2。它在结构上与玛瑙螺心脏兴奋肽（ACEP-1）相关，并且HCIP的末端氨基酸序列与FMRF酰胺家族肽的序列相同。3. 对合成的HCIP进行了心脏和神经元活性测试，发现它不仅对心室有抑制作用，而且对蜗牛中枢神经系统的内脏神经元也有抑制作用。免疫细胞化学研究表明它存在于内脏神经节和壁神经节中，在这些神经节中先前已鉴定出参与心跳调节的细胞。
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