BioTeC+, Chemical and Biochemical Process Technology and Control, Department of Chemical Engineering, KU Leuven, Ghent, Belgium.
School of Biological Sciences, Faculty of Science, Dublin Institute of Technology, Dublin, Ireland.
J Appl Microbiol. 2022 Sep;133(3):1919-1939. doi: 10.1111/jam.15684. Epub 2022 Jul 15.
This research aimed to develop and validate a cultivation and monitoring protocol that is suitable for a surrogate microbial community that accounts for the gut microbiota of the ileum of the small intestine.
Five bacterial species have been selected as representatives of the ileal gut microbiota and a general anaerobic medium (MS-BHI, as minimally supplemented brain heart infusion) has been constructed and validated against BCCM/LGM recommended and commercial media. Moreover, appropriate selective/differential media have been investigated for monitoring each ileal gut microbiota surrogate. Results showed that MS-BHI was highly efficient in displaying individual and collective behaviour of the ileal gut microbiota species, when compared with other types of media. Likewise, the selective/differential media managed to identify and describe the behaviour of their targeted species.
MS-BHI renders a highly efficient, inexpensive and easy-to-prepare cultivation and enumeration alternative for the surrogate ileal microbiota species. Additionally, the selective/differential media can identify and quantify the bacteria of the surrogate ileal microbial community.
The selected gut microbiota species can represent an in vitro ileal community, forming the basis for future studies on small intestinal microbiota. MS-BHI and the proposed monitoring protocol can be used as a standard for gut microbiota studies that utilize conventional microbiological techniques.
本研究旨在开发和验证一种适合于回肠肠道微生物群替代物的培养和监测方案。
选择了 5 种细菌作为回肠肠道微生物群的代表，并构建了一种通用的厌氧培养基（MS-BHI，即最小补充脑心浸液），并与 BCCM/LGM 推荐和商业培养基进行了验证。此外，还研究了适当的选择性/差异培养基，以监测每个回肠肠道微生物群替代物。结果表明，与其他类型的培养基相比，MS-BHI 能高度有效地显示回肠肠道微生物群物种的个体和集体行为。同样，选择性/差异培养基能够识别和描述其目标物种的行为。
MS-BHI 为替代回肠微生物群物种提供了一种高效、廉价且易于制备的培养和计数替代方法。此外，选择性/差异培养基可以识别和定量替代回肠微生物群落的细菌。
所选的肠道微生物群物种可以代表体外回肠群落，为未来的小肠微生物群研究奠定基础。MS-BHI 和拟议的监测方案可作为利用常规微生物学技术进行肠道微生物群研究的标准。
