College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, PR China.
Xi'an Dairy Cow Breeding Center, Xi'an Agriculture and Rural Affairs Bureau, Xi'an, Shaanxi, 710007, PR China.
Theriogenology. 2022 Sep 1;189:113-117. doi: 10.1016/j.theriogenology.2022.05.027. Epub 2022 Jun 17.
There are phospholipase and yolk agglutinase in the seminal plasma of dairy goat. The existence of these enzymes will hydrolyze lecithin in yolk into lysophosphatides and cause the change of pH value of diluent, resulting in toxic and side effects on sperm. In order to explore the effect of bovine seminal plasma replacing different doses of dairy goat seminal plasma (0%, 25%, 50%, 100%) on semen freezing quality of Guanzhong dairy goat, we used computer-aided sperm analysis (CASA) system, specific fluorescent probe detection technology, hypo osmotic swelling test (HOST), oxidation index determination and in vivo fertilization verification test to study the semen cryopreservation of Guanzhong dairy goat. The results showed that if we used bovine seminal plasma instead of 100% dairy goat seminal plasma, the sperm motility, plasma membrane functionality and acrosome integrity rate were the highest after thawing, reaching 67.04%, 38.12% and 68.83% respectively, which was significantly higher than that of other groups (P < 0.05); In terms of oxidation indexes, the values of SOD and GSH-PX in sperm of dairy goats were 199.78 U/mL and 133.55 U/L, respectively, which were significantly higher than those in other groups (P < 0.05), and the values of ROS and MDA were 397.58 U/mL and 5.16 nmol/L, respectively, which were significantly lower than those in other groups (P < 0.05); The results of artificial insemination also showed that using bovine seminal plasma instead of 100% of dairy goat seminal plasma could significantly improve the fertilization ability of sperm. The quality of frozen Guanzhong dairy goat sperm can be improved by using bovine seminal plasma instead of dairy goat seminal plasma and using bovine diluent.
奶山羊精液中含有磷脂酶和卵黄凝集素。这些酶的存在会将蛋黄中的卵磷脂水解成溶血磷脂，并导致稀释液 pH 值发生变化，从而对精子产生毒性和副作用。为了探讨用牛精液代替不同剂量（0%、25%、50%、100%）奶山羊精液对关中奶山羊精液冷冻质量的影响，我们采用计算机辅助精子分析（CASA）系统、特异荧光探针检测技术、低渗肿胀试验（HOST）、氧化指数测定及体内受精验证试验，对关中奶山羊精液进行了冷冻研究。结果表明，用牛精液代替 100%奶山羊精液时，解冻后精子的活力、质膜功能和顶体完整率最高，分别达到 67.04%、38.12%和 68.83%，显著高于其他组（P<0.05）；在氧化指标方面，奶山羊精子的 SOD 和 GSH-PX 值分别为 199.78 U/mL 和 133.55 U/L，显著高于其他组（P<0.05），ROS 和 MDA 值分别为 397.58 U/mL 和 5.16 nmol/L，显著低于其他组（P<0.05）；人工授精结果也表明，用牛精液代替 100%奶山羊精液可显著提高精子的受精能力。用牛精液代替奶山羊精液并用牛稀释液可提高冷冻关中奶山羊精子的质量。
