Pasciu Valeria, Nieddu Maria, Sotgiu Francesca Daniela, Baralla Elena, Berlinguer Fiammetta
Department of Veterinary Medicine, University of Sassari, 07100 Sassari, Italy.
Department of Medicine, Surgery and Pharmacy, University of Sassari, 07100 Sassari, Italy.
Animals (Basel). 2023 Jul 13;13(14):2300. doi: 10.3390/ani13142300.
The present review aims to provide an overview of the assay methods for the quantification of ROS and principal enzymatic antioxidants as biomarkers of oxidative stress in erythrocytes and spermatozoa of small domestic ruminants. A complete literature search was carried out in PubMed, Scopus and the World Wide Web using relevant keywords and focusing on the last five years (2018-2023). Among spectrophotometry, fluorometry and chemiluminescence, the most widely used method for ROS assay is fluorometry, probably because it allows to simultaneously assay several ROS, using different probes, with greater economic advantages. Regarding intracellular antioxidant enzymes, recent literature reports only spectrophotometric methods, many of which use commercial kits. The use of a less sensitive but cheapest method is suitable because both erythrocytes and spermatozoa samples are highly concentrated in domestic ruminant species. All methods considered in this review have been found to be appropriate; in general, the differences are related to their costs and sensitivity. Quantification of ROS and enzymatic antioxidant activity in erythrocytes and spermatozoa may find application in the study of the welfare and health status of small domestic ruminants for monitoring livestock production.
本综述旨在概述用于定量测定活性氧（ROS）和主要酶促抗氧化剂的分析方法，这些物质作为小型反刍家畜红细胞和精子中氧化应激的生物标志物。使用相关关键词并聚焦过去五年（2018 - 2023年），在PubMed、Scopus和万维网上进行了全面的文献检索。在分光光度法、荧光法和化学发光法中，最常用于ROS测定的方法是荧光法，这可能是因为它能够使用不同的探针同时检测多种ROS，具有更大的经济优势。关于细胞内抗氧化酶，最近的文献仅报道了分光光度法，其中许多使用商业试剂盒。使用不太灵敏但更便宜的方法是合适的，因为小型反刍家畜物种的红细胞和精子样本浓度都很高。本综述中考虑的所有方法都被认为是合适的；一般来说，差异与它们的成本和灵敏度有关。红细胞和精子中ROS和酶促抗氧化活性的定量测定可能在小型反刍家畜福利和健康状况研究中得到应用，以监测畜牧生产。
