Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
Curr Probl Cancer. 2022 Aug;46(4):100878. doi: 10.1016/j.currproblcancer.2022.100878. Epub 2022 Jun 11.
The significance of tumor infiltrating lymphocytes (TILs) in melanoma has been studied for a long time, but, to date, there has been insufficient research on TILs in sinonasal mucosal melanoma. The purpose of this study was to analyze the correlation between TILs and prognosis for Chinese patients with sinonasal mucosal melanoma, and to clarify the significance of TILs in prognosis. As a retrospective cohort study, 44 cases of malignant melanoma in head and neck mucosa were studied by immunohistochemical staining. The correlation between TIL classification (immune cell infiltration types), CD3, CD4, CD8, CD20, CD45, CD56, and CD68 positive cells, disease progression and prognosis for survival was analyzed. By pairing various factors, RNA sequencing and xCell analysis were performed in another 8 patients with different prognoses to further verify the expression of immune cell subsets in these patients. Immunohistochemistry and cell counting showed that the TIL classification and content of CD3, CD4, CD8, CD20, CD45, CD56, and CD68 positive cells were independent factors influencing progression-free survival, but there was no clear correlation with overall survival. RNA sequencing and xCell immunocyte analysis further confirmed the role of TILs in the prediction of disease progression. CD8+ T cells and natural killer T cells were highly expressed in patients with no disease progression, while Th2 T cells, macrophages and M2 macrophages were highly expressed in patients with disease progression. TILs can be used to predict the prognosis for patients with sinonasal mucosal melanoma. Different degrees and distributions of immune cell infiltration influence disease progression in patients with sinonasal mucosal melanoma. Patients with a diffuse distribution and a high density of infiltrating cells have a better prognosis. A high expression of CD8+ T cells and natural killer T cells, which have an immune killing effect, are beneficial in controlling progression of the disease.
肿瘤浸润淋巴细胞（TILs）在黑色素瘤中的意义已经研究了很长时间，但迄今为止，对鼻腔鼻窦黏膜黑色素瘤中的 TILs 研究还不够充分。本研究旨在分析 TILs 与中国鼻腔鼻窦黏膜黑色素瘤患者预后的相关性，阐明 TILs 在预后中的意义。本研究为回顾性队列研究，采用免疫组织化学染色对 44 例头颈部黏膜恶性黑色素瘤进行研究。分析 TIL 分类（免疫细胞浸润类型）、CD3、CD4、CD8、CD20、CD45、CD56、CD68 阳性细胞与疾病进展和预后生存的相关性。通过配对各种因素，对另外 8 例不同预后的患者进行 RNA 测序和 xCell 分析，以进一步验证这些患者免疫细胞亚群的表达。免疫组化和细胞计数显示，TIL 分类和 CD3、CD4、CD8、CD20、CD45、CD56、CD68 阳性细胞的含量是影响无进展生存期的独立因素，但与总生存期无明显相关性。RNA 测序和 xCell 免疫细胞分析进一步证实了 TIL 在疾病进展预测中的作用。无疾病进展患者的 CD8+T 细胞和自然杀伤 T 细胞表达较高，而疾病进展患者的 Th2 T 细胞、巨噬细胞和 M2 巨噬细胞表达较高。TIL 可用于预测鼻腔鼻窦黏膜黑色素瘤患者的预后。不同程度和分布的免疫细胞浸润影响鼻腔鼻窦黏膜黑色素瘤患者的疾病进展。弥漫性分布和浸润细胞密度较高的患者预后较好。具有免疫杀伤作用的 CD8+T 细胞和自然杀伤 T 细胞高表达有利于控制疾病进展。
