Centre for Nanosciences and Molecular Medicine, Amrita Vishwa Vidyapeetham, Kochi 682041, India.
Department of General Surgery, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi 682041, India.
Int J Biol Macromol. 2022 Aug 1;214:610-616. doi: 10.1016/j.ijbiomac.2022.06.113. Epub 2022 Jun 22.
The drug resistance is higher among Gram-negative bacteria and demands the usage of strong antibiotics which can in turn result in systemic toxicity. In the treatment of the chronic wounds harboring pathogenic Gram-negative bacteria, the demand for an antimicrobial product that can be topically administered has been on the rise. In an effort to address the above issue, we have developed Colistimethate sodium (a high-end antibiotic) loaded chitosan hydrogel and characterized. The prepared hydrogel is very stable and observed to be bio- and hemo-compatible in nature. The antibacterial activity of the prepared hydrogel was studied against both ATCC (American Type Culture Collection) strains and clinical isolates of Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae. The CMS incorporated hydrogel is also capable of inhibiting the biofilm formation. The developed hydrogel can be potentially being used for the treatment of Gram-negative bacterial infected wounds.
耐药性在革兰氏阴性菌中更高，需要使用强效抗生素，而这反过来又可能导致全身毒性。在治疗携带致病性革兰氏阴性菌的慢性伤口时，对可局部使用的抗菌产品的需求一直在上升。为了解决上述问题，我们开发了负载黏菌素钠（一种高端抗生素）的壳聚糖水凝胶并对其进行了特性描述。所制备的水凝胶非常稳定，在性质上表现出生物和血液相容性。研究了所制备的水凝胶对 ATCC（美国模式培养物集存库）菌株和临床分离的大肠杆菌、铜绿假单胞菌和肺炎克雷伯菌的抗菌活性。载 CMS 的水凝胶也能够抑制生物膜的形成。所开发的水凝胶可能可用于治疗革兰氏阴性菌感染的伤口。
