[Effect of the medium composition on the accumulation of 2-keto-D-gluconic acid in Pseudomonas putida cultures].

The effect of the composition of the culture medium and the age of the culture on the activities of the enzymes involved in accumulation of 2-ketogluconic acid by Pseudomonas putida was studied. The activities of glucose and gluconate dehydrogenases that are responsible for direct oxidation of glucose to 2-ketogluconic acid, were 2-3 times higher during the active growth of the culture than in the stationary phase. The activities of 2-ketogluconokinase and 2-keto-6-phosphogluconate reductase, enzymes converting 2-ketogluconic acid, increased 2-4-fold on the glucose exhausting. The latter enzymes were not active when the culture was grown on nitrogen or phosphorus deficient media, and in this case 2-ketogluconic acid was accumulated in the medium.