SR5AL 在 Blakeslea trispora 中的番茄红素生物合成中作为关键调控基因。
SR5AL serves as a key regulatory gene in lycopene biosynthesis by Blakeslea trispora.
机构信息
College of Life Sciences, Henan Normal University, Xinxiang, 453007, China.
Henan International Joint Laboratory of Agricultural Microbial Ecology and Technology (Henan Provincial Department of Science and Technology), Henan Normal University, Xinxiang, 453007, China.
出版信息
Microb Cell Fact. 2022 Jun 25;21(1):126. doi: 10.1186/s12934-022-01853-x.
BACKGROUND
Trisporic acids are considered to be key regulators of carotenoid biosynthesis and sexual reproduction in zygomycetes, but the mechanisms underlying this regulation have not been fully elucidated.
RESULTS
In this study, the relationships between trisporic acids and lycopene synthesis were investigated in Blakeslea trispora. The lycopene concentration in single fermentation by the (-) strain with the addition of 24 μg/L trisporic acids was slightly higher than that observed in mated fermentation. After transcriptomic analysis, a steroid 5α-reductase-like gene, known as SR5AL in B. trispora, was first reported. 5α-Reductase inhibitors reduced lycopene biosynthesis and downregulated the expression of sex determination and carotenoid biosynthesis genes. Overexpression of the SR5AL gene upregulated these genes, regardless of whether trisporic acids were added.
CONCLUSION
These findings indicated that the SR5AL gene is a key gene associated with the response to trisporic acids.
背景
三烯酸被认为是接合菌中类胡萝卜素生物合成和有性生殖的关键调节剂，但这种调节的机制尚未完全阐明。
结果
本研究在三孢布拉氏霉中研究了三烯酸与番茄红素合成的关系。添加 24μg/L 三烯酸的（-）菌株进行单一发酵时，番茄红素浓度略高于交配发酵时的浓度。经过转录组分析，首次报道了一种甾醇 5α-还原酶样基因，在三孢布拉氏霉中称为 SR5AL。5α-还原酶抑制剂降低了番茄红素的生物合成，并下调了性别决定和类胡萝卜素生物合成基因的表达。无论是否添加三烯酸，SR5AL 基因的过表达都上调了这些基因。
结论
这些发现表明，SR5AL 基因是与三烯酸反应相关的关键基因。
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