College of Animal Science and Technology, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an 271018, Shandong, China.
College of Life Sciences, Shandong Agricultural University, Tai'an 271018, Shandong, China.
Poult Sci. 2022 Aug;101(8):101977. doi: 10.1016/j.psj.2022.101977. Epub 2022 May 27.
In order to enrich the knowledge of chicken transcriptomic response to Salmonella enterica serovar Enteritidis infection, 2-day-old chicks were orally inoculated with this bacteria (1.0 × 10 cfu/mL), and then the cecum tissues of 3 days post-inoculation were utilized for RNA sequencing (6 replicates each for treatment group and control group). After analysis, we found a variety of inflammatory genes were triggered at the mRNA level upon infection. Notably, the expression profiles at the miRNA level and the isomiR level were heterogeneous. Certain isomiRs of chicken miR-146b-5p were significantly increased by more than 2 times compared to control (Padj < 0.05). Combining the bioinformatics prediction, transcriptome data and RT-qPCR results, we deduced that the isomiRs of chicken miR-146b-5p might act to sustain the RIG-I-like receptor signaling and type I interferon induction by repressing USP3 transcript. Our findings provide a new perspective on the regulatory function of miR-146b-5p and facilitate the study of isomiRs.
为了丰富鸡对肠炎沙门氏菌感染的转录组反应知识，将 2 日龄小鸡经口接种该细菌（1.0×10 cfu/mL），然后在接种后 3 天使用盲肠组织进行 RNA 测序（每组 6 个重复）。分析后，我们发现感染会在 mRNA 水平上引发多种炎症基因。值得注意的是，miRNA 水平和 isomiR 水平的表达谱存在异质性。与对照组相比，鸡 miR-146b-5p 的某些 isomiR 增加了 2 倍以上（Padj < 0.05）。通过生物信息学预测、转录组数据和 RT-qPCR 结果相结合，我们推断鸡 miR-146b-5p 的 isomiR 可能通过抑制 USP3 转录来维持 RIG-I 样受体信号和 I 型干扰素诱导。我们的研究结果为 miR-146b-5p 的调控功能提供了新的视角，并促进了对 isomiR 的研究。
