Liu Yi, Sun Dongyue, Xu Congcong, Liu Xiaoyong, Tang Min, Ying Shijia
School of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, China.
Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
Front Vet Sci. 2024 Jul 22;11:1417244. doi: 10.3389/fvets.2024.1417244. eCollection 2024.
Amidst rising global temperatures, chronic heat stress (CHS) is increasingly problematic for the poultry industry. While mammalian CHS responses are well-studied, avian-specific research is lacking. This study uses in-depth transcriptome sequencing to evaluate the pulmonary response of Cherry Valley ducks to CHS at ambient temperatures of 20°C and a heat-stressed 29°C. We detailed the CHS-induced gene expression changes, encompassing mRNAs, lncRNAs, and miRNAs. Through protein-protein interaction network analysis, we identified central genes involved in the heat stress response-, , , , , , and . Subsequent functional enrichment analysis of the differentially expressed genes and RNA targets revealed significant engagement in immune responses and regulatory processes. KEGG pathway analysis underscored crucial immune pathways, specifically those related to intestinal IgA production and Toll-like receptor signaling, as well as Salmonella infection and calcium signaling pathways. Importantly, we determined six miRNAs-miR-146, miR-217, miR-29a-3p, miR-10926, miR-146b-5p, and miR-17-1-3p-as potential key regulators within the ceRNA network. These findings enhance our comprehension of the physiological adaptation of ducks to CHS and may provide a foundation for developing strategies to improve duck production under thermal stress.
在全球气温不断上升的背景下，慢性热应激（CHS）对家禽业来说问题日益严重。虽然对哺乳动物的慢性热应激反应已有充分研究，但针对禽类的相关研究却很缺乏。本研究采用深度转录组测序技术，评估樱桃谷鸭在20°C环境温度和29°C热应激温度下对慢性热应激的肺部反应。我们详细阐述了慢性热应激诱导的基因表达变化，包括mRNA、lncRNA和miRNA。通过蛋白质-蛋白质相互作用网络分析，我们确定了参与热应激反应的核心基因，即 、 、 、 、 、 以及 。随后对差异表达基因和RNA靶点进行的功能富集分析表明，它们在免疫反应和调节过程中发挥了重要作用。KEGG通路分析强调了关键的免疫通路，特别是与肠道IgA产生和Toll样受体信号传导相关的通路，以及沙门氏菌感染和钙信号通路。重要的是，我们确定了六种miRNA——miR-146、miR-217、miR-29a-3p、miR-10926、miR-146b-5p和miR-17-1-3p——作为ceRNA网络中的潜在关键调节因子。这些发现增强了我们对鸭子适应慢性热应激生理机制的理解，并可能为制定在热应激条件下提高鸭子生产性能的策略提供基础。
