泰国小型家庭奶牛场中用作奶牛饲料的玉米残渣中黄曲霉毒素 B 浓度受储存条件和霉菌类型的影响。

Impact of Storage Conditions and Mold Types on Aflatoxin B Concentration in Corn Residue used as Dairy Feed in Small Holder Dairy Farms, Thailand.

机构信息

Research center of Producing and Development of Products and Innovations for Animal Health and Production, Faculty of Veterinary Medicine, Chiang Mai University.

Department of Physiology, Faculty of Science, Mahidol University.

出版信息

Biocontrol Sci. 2022;27(2):99-105. doi: 10.4265/bio.27.99.

DOI:10.4265/bio.27.99

PMID:35753798

Abstract

The aims of this study were to determine the impact of storage practice and mold types on mold growth and aflatoxin B (AFB) concentration in corn residue from local seed corn plants, the main roughage source of dairy farms in the northern region in Thailand. A total of 223 samples from 2 types of corn residue - dried and wet - were collected. Mold contamination was determined by spread plate technique, and aflatoxin B (AFB) quantification was performed by a commercial enzyme-linked immunosorbent assay. Multivariate linear models were created to determine factors associated with fungal quantity and AFB concentration. Results showed that the presence of Cladosporium spp. in the samples was associated with a lower risk of AFB1 contamination (P<0.05). In addition, appropriate storage practices, e.g. keeping feeds under a roof and using floor canvas under feed piles, gave lower risk of mold contamination and decreasing AFB contamination.

摘要

本研究旨在确定储存方式和霉菌类型对来自泰国北部地区奶牛场主要粗饲料来源的本地种子玉米植株玉米残渣中霉菌生长和黄曲霉毒素 B（AFB）浓度的影响。共采集了 223 个来自干玉米残渣和湿玉米残渣的样本。采用平板扩散技术测定霉菌污染情况，采用商业酶联免疫吸附试验定量测定黄曲霉毒素 B（AFB）。创建多元线性模型以确定与真菌数量和 AFB 浓度相关的因素。结果表明，样本中存在枝孢属（Cladosporium spp.）与 AFB1 污染风险较低有关（P<0.05）。此外，适当的储存方式，如将饲料存放在屋檐下并在饲料堆下使用地板帆布，可以降低霉菌污染和 AFB 污染的风险。