咖啡因挑战下血脑屏障通透性的变化。
Blood-brain barrier permeability in response to caffeine challenge.
机构信息
The Russell H. Morgan Department of Radiology & Radiological Science, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Department of Radiology, New York University, New York, New York, USA.
出版信息
Magn Reson Med. 2022 Nov;88(5):2259-2266. doi: 10.1002/mrm.29355. Epub 2022 Jun 26.
PURPOSE
Caffeine is known to alter brain perfusion by acting as an adenosine antagonist, but its effect on blood-brain barrier (BBB) permeability is not fully elucidated. This study aimed to dynamically monitor BBB permeability to water after a single dose of caffeine tablet using a non-contrast MRI technique.
METHODS
Ten young healthy volunteers who were not regular coffee drinkers were studied. The experiment began with a pre-caffeine measurement, followed by four measurements at the post-caffeine stage. Water-extraction-with-phase-contrast-arterial-spin-tagging (WEPCAST) MRI was used to assess the time dependence of BBB permeability to water following the ingestion of 200 mg caffeine. Other cerebral physiological parameters including cerebral blood flow (CBF), venous oxygenation (Y ), and cerebral metabolic rate of oxygen (CMRO ) were also examined. The relationships between cerebral physiological parameters and time were studied with mixed-effect models.
RESULTS
It was found that, after caffeine ingestion, CBF and Y showed a time-dependent decrease (p < 0.001), while CMRO did not change significantly. The fraction of arterial water crossing the BBB (E) showed a significant increase (p < 0.001). In contrast, the permeability-surface-area product (PS), i.e., BBB permeability to water, remained constant (p = 0.94). Additionally, it was observed that changes in physiological parameters were non-linear with regard to time and occurred at as early as 9 min after caffeine tablet ingestion.
CONCLUSION
These results suggest an unchanged BBB permeability despite alterations in perfusion during a vasoconstrictive caffeine challenge.
目的
咖啡因通过作为腺苷拮抗剂来改变脑灌注，但它对血脑屏障（BBB）通透性的影响尚未完全阐明。本研究旨在使用非对比 MRI 技术，单次服用咖啡因片后动态监测 BBB 对水的通透性。
方法
研究了 10 名不常喝咖啡的年轻健康志愿者。实验开始前进行了一次咖啡因前测量，然后在咖啡因后阶段进行了四次测量。使用水提取相位对比动脉自旋标记（WEPCAST）MRI 来评估在摄入 200mg 咖啡因后，水通过 BBB 的通透性随时间的变化。还检查了包括脑血流（CBF）、静脉氧合（Y）和脑氧代谢率（CMRO）在内的其他脑生理参数。使用混合效应模型研究了脑生理参数与时间之间的关系。
结果
发现，在摄入咖啡因后，CBF 和 Y 表现出时间依赖性下降（p<0.001），而 CMRO 没有明显变化。穿过 BBB 的动脉水分数（E）显著增加（p<0.001）。相比之下，渗透性表面积乘积（PS），即水通过 BBB 的通透性保持不变（p=0.94）。此外，还观察到生理参数的变化与时间呈非线性关系，早在摄入咖啡因片后 9 分钟就出现了变化。
结论
这些结果表明，在血管收缩性咖啡因挑战期间，尽管灌注发生变化，但 BBB 通透性保持不变。
Zotero 插件