Rao Jie, Li Siyu, Wang Xinyu, Cheng Qi, Ji Yu, Fu Wenwen, Huang Hui, Shi Ling, Wu Xiaorong
Department of Ophthalmology, The First Affiliated Hospital of Nanchang University, Nanchang, China.
Front Med (Lausanne). 2022 Jun 9;9:851808. doi: 10.3389/fmed.2022.851808. eCollection 2022.
This study aims to discuss the function mechanism of regulatory T cells and its subsets in the pathogenic process of myasthenia gravis by contracting the activation levels of those cells in peripheral blood among healthy people, patients with ocular myasthenia gravis (oMG) and patients with generalized myasthenia gravis (gMG).
Healthy people, newly diagnosed oMG patients, and gMG patients were enrolled in this study. The percentage of the CD3CD4CD25 Treg cells, CD3CD4CD25Foxp3 Treg cells, CD3CD4CD25Foxp3 CD45RAaTreg cells, CD3CD4CD25Foxp3CD45RAn-sTreg cells, and CD3CD4CD25 Foxp3CD45RArTreg cells in the peripheral blood were examined by flow cytometry. And then analyzed the differences of Treg cells and its subsets among the study members.
The percentage of the CD4CD25Treg cells in the peripheral blood of oMG patients and gMG patients were both lower than that of healthy people ( < 0.05), the percentage of patients with oMG had no distinct difference with that of patients with gMG ( = 0.475), however. Also, the percentage of CD3CD4CD25Foxp3Treg cells in the oMG and gMG patients' group were both lower than that of healthy group. And the percentage of CD25Foxp3Treg cells in the peripheral blood of patients with oMG and healthy people were both higher than that of patients with gMG ( < 0.05). The percentage of rTreg in the CD3CD4CD25Treg of the peripheral blood for both gMG and oMG patients' group were lower than healthy group ( < 0.05), but there was no statistical significance between the oMG and gMG patients' group ( = 0.232). The percentage of the aTreg cells in the CD3CD4CD25Treg cells of the peripheral blood for the oMG patients was higher than that of gMG patients ( < 0.05), but both of them were lower than healthy group ( < 0.05). The percentage of n-sTreg cells in the peripheral blood descended among the gMG patients' group, oMG patients' group, and healthy group ( < 0.05).
The changes in the number and function of Treg cells and its subsets can cause the impairment of negative immune regulation, which may mediate the triggering of oMG and its progression to gMG.
本研究旨在通过对比健康人、眼肌型重症肌无力（oMG）患者和全身型重症肌无力（gMG）患者外周血中调节性T细胞及其亚群的激活水平，探讨其在重症肌无力发病过程中的作用机制。
本研究纳入健康人、新诊断的oMG患者和gMG患者。采用流式细胞术检测外周血中CD3CD4CD25 Treg细胞、CD3CD4CD25Foxp3 Treg细胞、CD3CD4CD25Foxp3 CD45RAaTreg细胞、CD3CD4CD25Foxp3CD45RAn-sTreg细胞和CD3CD4CD25 Foxp3CD45RArTreg细胞的百分比。然后分析研究对象中Treg细胞及其亚群的差异。
oMG患者和gMG患者外周血中CD4CD25Treg细胞的百分比均低于健康人（P<0.05），然而，oMG患者与gMG患者的百分比无明显差异（P = 0.475）。此外，oMG和gMG患者组中CD3CD4CD25Foxp3Treg细胞的百分比均低于健康组。oMG患者和健康人外周血中CD25Foxp3Treg细胞的百分比均高于gMG患者（P<0.05）。gMG和oMG患者组外周血CD3CD4CD25Treg中rTreg的百分比均低于健康组（P<0.05），但oMG和gMG患者组之间无统计学意义（P = 0.232）。oMG患者外周血CD3CD4CD25Treg细胞中aTreg细胞的百分比高于gMG患者（P<0.05），但两者均低于健康组（P<0.05）。gMG患者组、oMG患者组和健康组外周血中n-sTreg细胞的百分比均下降（P<0.05）。
Treg细胞及其亚群数量和功能的变化可导致负性免疫调节受损，这可能介导oMG的触发及其向gMG的进展。
