Pina-Coronado Clara, Martínez-Sobrino Álvaro, Gutiérrez-Gálvez Laura, Del Caño Rafael, Martínez-Periñán Emiliano, García-Nieto Daniel, Rodríguez-Peña Micaela, Luna M, Milán-Rois Paula, Castellanos Milagros, Abreu Melanie, Cantón Rafael, Galán Juan Carlos, Pineda Teresa, Pariente Félix, Somoza Álvaro, García-Mendiola Tania, Miranda Rodolfo, Lorenzo Encarnación
Departamento de Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid, Madrid 28049, Spain.
Departamento de Química Física y Termodinámica Aplicada e Instituto Universitario de Nanoquímica, Universidad de Córdoba, Córdoba 14014, Spain.
Sens Actuators B Chem. 2022 Oct 15;369:132217. doi: 10.1016/j.snb.2022.132217. Epub 2022 Jun 17.
The development of DNA-sensing platforms based on new synthetized Methylene Blue functionalized carbon nanodots combined with different shape gold nanostructures (AuNs), as a new pathway to develop a selective and sensitive methodology for SARS-CoV-2 detection is presented. A mixture of gold nanoparticles and gold nanotriangles have been synthetized to modify disposable electrodes that act as an enhanced nanostructured electrochemical surface for DNA probe immobilization. On the other hand, modified carbon nanodots prepared to contain Methylene Blue (MB-CDs) are used as electrochemical indicators of the hybridization event. These MB-CDs, due to their structure, are able to interact differently with double and single-stranded DNA molecules. Based on this strategy, target sequences of the SARS-CoV-2 virus have been detected in a straightforward way and rapidly with a detection limit of 2.00 aM. Moreover, this platform allows the detection of the SARS-CoV-2 sequence in the presence of other viruses, and also a single nucleotide polymorphism (SNPs). The developed approach has been tested directly on RNA obtained from nasopharyngeal samples from COVID-19 patients, avoiding any amplification process. The results agree well with those obtained by RT-qPCR or reverse transcription quantitative polymerase chain reaction technique.
本文介绍了一种基于新合成的亚甲基蓝功能化碳纳米点与不同形状金纳米结构（AuNs）相结合的DNA传感平台的开发，这是开发一种用于检测新型冠状病毒（SARS-CoV-2）的选择性和灵敏方法的新途径。已合成金纳米颗粒和金纳米三角形的混合物，以修饰用作DNA探针固定化增强纳米结构电化学表面的一次性电极。另一方面，制备的含有亚甲基蓝的修饰碳纳米点（MB-CDs）用作杂交事件的电化学指示剂。这些MB-CDs由于其结构，能够与双链和单链DNA分子发生不同的相互作用。基于此策略，已直接且快速地检测到新型冠状病毒的靶序列，检测限为2.00 aM。此外，该平台能够在存在其他病毒以及单核苷酸多态性（SNP）的情况下检测新型冠状病毒序列。所开发的方法已直接在从新冠肺炎患者鼻咽样本中获得的RNA上进行测试，避免了任何扩增过程。结果与通过逆转录定量聚合酶链反应（RT-qPCR）技术获得的结果非常吻合。
