Division of Cardiothroracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
Comput Intell Neurosci. 2022 Jun 16;2022:5168886. doi: 10.1155/2022/5168886. eCollection 2022.
Ultrasound irradiation is now the best method for evaluating benign and malignant tumor nodules. Chemotherapy has always played an important role in the treatment of malignant tumors. With the large-scale application of chemotherapy drugs, the problem of multidrug resistance of tumors has become more and more prominent, which has become one of the difficulties in tumor chemotherapy. This study mainly explores the antitumor proliferation and related mechanisms of ultrasound irradiation combined with safflower yellow. The breast cancer cell line 4T1 derived from BALB/c mice was selected. BALB/c is an albino laboratory mouse, which, like many commonly used sublines, originated from Mus musculus. BALB/c mice have been bred for more than 200 generations in research institutions around the world and are widely used in animal experiments in immunology and physiology. When the cell proliferation reached 80%-90% of the bottom area of the culture flask, it was resuspended, passaged, frozen, and resuscitated according to experimental needs. The 4T1 breast cancer cell line was cultured by conventional methods. 4T1 breast cancer cells in the logarithmic proliferation phase were collected. After 0.25% was digested with pancreatin, it was washed twice with PBS to adjust the concentration to 1 × 10/mL. A 0.1 mL tumor cell suspension was subcutaneously inoculated on the edge of the mouse chest, thereby establishing a breast cancer model of BALB/c mice. After 6-15 days, the tumor volume grew rapidly and became larger. When the length of the tumor is 2.5 × 2.5, the modeling is successful. Ultrasound-targeted microbubble destruction technology, as a novel drug delivery method with high efficiency and low toxicity, can form transient pores (sonoporation effect) on the cell surface, widen the intercellular space, and increase the membrane permeability, and thus effectively. The transport of drugs, genes, proteins, etc., is promoted to target organs and tissues. Tumor-forming mice were randomly divided into the following four groups: control group, safflower yellow group, ultrasound irradiation group, and ultrasound irradiation combined with safflower yellow group. From the second day of inoculation to the end of the experiment, the body weight of the mice successfully inoculated with 4T1 cells was measured every day; from the 5th day, tumors in each group were calculated body volume and tumor inhibition rate (TIR) of each group. The combined treatment group has a higher tumor inhibition rate than the ultrasound irradiation group, and the difference is statistically significant ( < 0.05). Ultrasound irradiation combined with safflower yellow pigment can effectively inhibit tumor proliferation, maintain, or even improve the efficacy of chemotherapy, thereby improving the patient's tolerance to chemotherapy.
超声辐射是评估良恶性肿瘤结节的最佳方法。化疗一直是恶性肿瘤治疗的重要手段。随着化疗药物的大规模应用，肿瘤的多药耐药问题越来越突出，成为肿瘤化疗的难点之一。本研究主要探讨超声辐射联合红花黄色素对肿瘤增殖的抑制作用及其相关机制。选用 BALB/c 小鼠来源的乳腺癌细胞系 4T1。BALB/c 是一种白化实验鼠，与许多常用的亚系一样，起源于 Mus musculus。BALB/c 小鼠已在世界各地的研究机构中繁殖了 200 多代，广泛应用于免疫和生理学动物实验。当细胞增殖达到培养瓶底面积的 80%-90%时，根据实验需要进行悬浮、传代、冷冻和复苏。常规方法培养 4T1 乳腺癌细胞。收集对数增殖期的 4T1 乳腺癌细胞，用 0.25%胰蛋白酶消化后，用 PBS 洗 2 次，调整浓度至 1×10/mL。皮下接种 0.1ml 肿瘤细胞悬液于小鼠胸部边缘，建立 BALB/c 小鼠乳腺癌模型。6-15 天后，肿瘤体积迅速生长，体积增大。当肿瘤的长度为 2.5×2.5 时，建模成功。超声靶向微泡破坏技术作为一种高效低毒的新型药物输送方法，可在细胞表面形成瞬时孔（声孔效应），扩大细胞间隙，增加细胞膜通透性，从而有效促进药物、基因、蛋白质等的输送。到达靶器官和组织。将成瘤小鼠随机分为以下四组：对照组、红花黄色素组、超声辐照组、超声辐照联合红花黄色素组。从接种后第二天到实验结束，每天测量成功接种 4T1 细胞的小鼠的体重；从第 5 天起，计算每组肿瘤的体积和肿瘤抑制率（TIR）。联合治疗组的肿瘤抑制率高于超声照射组，差异有统计学意义（ < 0.05）。超声辐照联合红花黄色素能有效抑制肿瘤增殖，维持甚至提高化疗疗效，从而提高患者对化疗的耐受性。
