Mashela Phatu William, Pofu Kgabo Martha, Bopape-Mabapa Moshibudi Paulina
Department of Plant Production, Soil Science and Agricultural Engineering, Green Biotechnologies Research Centre of Excellence, University of Limpopo, Polokwane, South Africa.
Front Microbiol. 2022 Jun 10;13:863808. doi: 10.3389/fmicb.2022.863808. eCollection 2022.
The cost and environment concerns of existing drench-application technologies of cucurbitacin phytonematicides resulted in conceptualization of the priming-and-drying (PAD) technology of seeds with hypogeal germination. The preliminary observations suggested that the PAD technology improved seed germination, plant growth, and vigor in pea (), with limited information on suppression of root-knot ( species) nematodes. Post-soaking pea seeds in geometric concentrations of Nemarioc-AL and Nemafric-BL phytonematicides, seedlings were raised in greenhouse and on microplot experiments during 2019 and validated in 2020. At 60 days after inoculation with 300 eggs + second-stage juveniles (J2) of seasonal data were pooled. Gall rating, eggs in root, and J2 in root vs. Nemarioc-AL phyto nematicide in greenhouse and on microplot exhibited negative quadratic relations, with models explained by 80-85% and 89-94% associations, respectively. Similarly, for the respective sites negative quadratic models for nematode variables vs. Nemafric-BL phytonematicide were explained by 82-93% and 90-94% associations, respectively. In conclusion, pea seed remnants belowground gradually released cucurbitacins into the rhizosphere throughout the growing period, thereby suppressing nematode population densities, and therefore, the PAD technology has the potential for assessment in a large-scale application of cucurbitacin phytonematicides for pea production.
现有葫芦素杀线虫剂浸施技术的成本和环境问题促使人们构思了针对地下发芽种子的引发干燥(PAD)技术。初步观察表明,PAD技术提高了豌豆的种子发芽率、植株生长和活力(),但关于抑制根结(种)线虫的信息有限。将豌豆种子浸泡在几何浓度的Nemarioc-AL和Nemafric-BL杀线虫剂中后,于2019年在温室和微区试验中培育幼苗,并于2020年进行验证。接种300个虫卵+第二季幼虫(J2)后60天,将季节性数据汇总。温室和微区中,根结线虫评级、根内虫卵和根内J2与Nemarioc-AL杀线虫剂呈负二次关系,模型的关联度分别为80-85%和89-94%。同样,在各个试验点,线虫变量与Nemafric-BL杀线虫剂的负二次模型的关联度分别为82-93%和90-94%。总之,地下的豌豆种子残渣在整个生长期间逐渐将葫芦素释放到根际,从而抑制线虫种群密度,因此,PAD技术有潜力用于评估葫芦素杀线虫剂在豌豆生产中的大规模应用。