Hepatopancreatobiliary Surgery Department, Kunming Medical University, 650101 Kunming, China.
Rheumatology and Immunology Department, Affiliated Hospital of Southwest Medical University, 646000 Luzhou, China.
Dis Markers. 2022 Jun 15;2022:8605621. doi: 10.1155/2022/8605621. eCollection 2022.
Dendritic cells (DC) initiate the immune response in the body. They can stimulate T cell activation, proliferation, and differentiation and ultimately participate in the immune response and the immune tolerance response. The purpose of this study was to coculture DCs and T cells and subcutaneously inject DCs transfected with miR-let-7i into rhesus monkey transplantations to verify the role of miR-let-7i in allograft immune tolerance. In vitro studies found that the expression of miR-let-7i was upregulated after inducing the maturation of DCs. The low expression of miR-let-7i inhibited the maturation of DCs, promoted the differentiation of T cells into T helper T cells 2 (Th2), and inhibited T helper T cell 1- (Th1-) driven rejection. In vivo studies also obtained similar results, and subcutaneous injection of DCs transfected with miR-let-7i inhibitor prolonged the survival time of allogeneic skin transplantation. Therefore, we conclude that inhibition of miR-let-7i inhibits DC maturation and improves the tolerance of grafted skin.
树突状细胞（DC）在体内启动免疫反应。它们可以刺激 T 细胞的激活、增殖和分化，最终参与免疫反应和免疫耐受反应。本研究的目的是将 DC 和 T 细胞共培养，并将转染 miR-let-7i 的 DC 皮下注射到恒河猴移植中，以验证 miR-let-7i 在同种异体免疫耐受中的作用。体外研究发现，诱导 DC 成熟后 miR-let-7i 的表达上调。miR-let-7i 的低表达抑制 DC 的成熟，促进 T 细胞向辅助性 T 细胞 2（Th2）分化，并抑制辅助性 T 细胞 1-（Th1-）驱动的排斥。体内研究也得到了类似的结果，转染 miR-let-7i 抑制剂的 DC 皮下注射延长了同种异体皮肤移植的存活时间。因此，我们得出结论，抑制 miR-let-7i 抑制 DC 成熟并提高移植物皮肤的耐受性。
