College of Pharmacy, Shri Indra Ganesan Institute of Medical Science, Manikandam, Tiruchirapalli, 620012 Tamil Nadu, India.
Department of Anesthesia Technology, College of Applied Medical Sciences in Jubail, Imam Abdulrahman Bin Faisal University, P.O. Box 4030, Jubail, Saudi Arabia.
Biomed Res Int. 2022 Jun 17;2022:4094132. doi: 10.1155/2022/4094132. eCollection 2022.
The core objectives of the research were to prepare 5-fluorouracil nanoemulsion (FU-NE) and to evaluate the physiochemical properties and to study the i antiproliferation in HepG2 cell lines. The physiochemical parameters determined were compatibility, particle size (PS), polydispersity index (PDI), zeta potential (ZP), density, surface tension (ST), pH, viscosity, release of FU, cytotoxicity, and apoptosis study. The prepared FU-NE3 was stable, sterile, and homogeneous. On the HepG2 (120 g.mL) cells, cytotoxicity was obtained at IC concentration. Apoptosis examination by AO/EBand Hoechst staining shows that the majority of cell demise was caused by apoptosis, with a tiny fraction of necrosis. Hence, this investigation concluded that the developed FU-NE has now desirable characteristics for drug delivery to the cancer cell and may be screened for the colorectal anticancer activity.
本研究的核心目标是制备 5-氟尿嘧啶纳米乳（FU-NE），并评估其理化性质以及在 HepG2 细胞系中的抗增殖作用。测定的理化参数包括相容性、粒径（PS）、多分散指数（PDI）、Zeta 电位（ZP）、密度、表面张力（ST）、pH 值、黏度、FU 的释放、细胞毒性和凋亡研究。制备的 FU-NE3 稳定、无菌且均匀。在 HepG2（120 g.mL）细胞上，在 IC 浓度时获得了细胞毒性。AO/EB 和 Hoechst 染色的凋亡检测表明，大多数细胞死亡是由凋亡引起的，只有一小部分是坏死。因此，本研究得出结论，开发的 FU-NE 具有将药物递送到癌细胞的理想特性，并且可能被筛选用于结直肠癌的抗癌活性。
