• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

作为潜在的活疫苗，共同表达针对实验性皮肤利什曼病的不同唾液腺蛋白，在 BALB/c 小鼠模型中。

as Potential Live Vaccine Co-Expressing Distinct Salivary Gland Proteins Against Experimental Cutaneous Leishmaniasis in BALB/c Mice Model.

机构信息

Department of Medical Biotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.

Department of Immunotherapy and Leishmania Vaccine Research, Pasteur Institute of Iran, Tehran, Iran.

出版信息

Front Immunol. 2022 Jun 10;13:895234. doi: 10.3389/fimmu.2022.895234. eCollection 2022.

DOI:10.3389/fimmu.2022.895234
PMID:35757692
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9226313/
Abstract

Leishmaniasis is a neglected vector-borne disease caused by parasites transmitted through the infected sand flies bite. Current treatments are limited, partly due to their high cost and significant adverse effects, and no human vaccine is yet available. Sand flies saliva has been examined for their potential application as an anti- vaccine. The salivary protein, PpSP15, was the first protective vaccine candidate against . Additionally, PsSP9 was already introduced as a highly immunogenic salivary protein against . Herein, we aimed to develop an effective multivalent live vaccine to control Cutaneous Leishmaniasis induced by two main species, and . Hence, the two above-mentioned salivary proteins using T2A linker were incorporated inside the genome as a safe live vector. Then, the immunogenicity and protective effects of recombinant co-expressing PpSP15 and PsSP9 were evaluated in pre-treated BALB/c mice with CpG against and . Following the cytokine assays, parasite burden and antibody assessment at different time-points at pre and post-infection, promising protective Th1 immunity was obtained in vaccinated mice with recombinant co-expressing PpSP15 and PsSP9. This is the first study demonstrating the potency of a safe live vaccine based on the combination of different salivary proteins against the infectious challenge with two different species of .

摘要

利什曼病是一种被忽视的媒介传播疾病，由寄生虫通过受感染的沙蝇叮咬传播。目前的治疗方法有限，部分原因是其成本高且副作用大，而且尚未有针对人类的疫苗。沙蝇唾液已被研究用于潜在的疫苗应用。唾液蛋白 PpSP15 是第一个针对 的保护性疫苗候选物。此外，PsSP9 已被引入作为一种针对 的高度免疫原性唾液蛋白。在此，我们旨在开发一种有效的多价活疫苗来控制由 和 两种主要物种引起的皮肤利什曼病。因此，使用 T2A 接头将上述两种唾液蛋白掺入 基因组内作为安全的活载体。然后，用 CpG 预处理 BALB/c 小鼠，评估重组 同时表达 PpSP15 和 PsSP9 的免疫原性和保护效果，以对抗 和 。在细胞因子分析后，在感染前和感染后不同时间点进行寄生虫负荷和抗体评估，在接种重组 同时表达 PpSP15 和 PsSP9 的小鼠中获得了有希望的保护性 Th1 免疫。这是第一项研究，证明了基于不同唾液蛋白组合的安全活疫苗针对两种不同物种的传染性挑战的效力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a33e/9226313/0f4a781f9f9b/fimmu-13-895234-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a33e/9226313/1660fb51805b/fimmu-13-895234-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a33e/9226313/b1e6c37c17e2/fimmu-13-895234-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a33e/9226313/3b430d5ec868/fimmu-13-895234-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a33e/9226313/19545584e854/fimmu-13-895234-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a33e/9226313/58c1341bf944/fimmu-13-895234-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a33e/9226313/f39312942073/fimmu-13-895234-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a33e/9226313/b2de0c3fdd65/fimmu-13-895234-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a33e/9226313/12661290e977/fimmu-13-895234-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a33e/9226313/0f4a781f9f9b/fimmu-13-895234-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a33e/9226313/1660fb51805b/fimmu-13-895234-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a33e/9226313/b1e6c37c17e2/fimmu-13-895234-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a33e/9226313/3b430d5ec868/fimmu-13-895234-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a33e/9226313/19545584e854/fimmu-13-895234-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a33e/9226313/58c1341bf944/fimmu-13-895234-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a33e/9226313/f39312942073/fimmu-13-895234-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a33e/9226313/b2de0c3fdd65/fimmu-13-895234-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a33e/9226313/12661290e977/fimmu-13-895234-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a33e/9226313/0f4a781f9f9b/fimmu-13-895234-g009.jpg

相似文献

1
as Potential Live Vaccine Co-Expressing Distinct Salivary Gland Proteins Against Experimental Cutaneous Leishmaniasis in BALB/c Mice Model.作为潜在的活疫苗，共同表达针对实验性皮肤利什曼病的不同唾液腺蛋白，在 BALB/c 小鼠模型中。
Front Immunol. 2022 Jun 10;13:895234. doi: 10.3389/fimmu.2022.895234. eCollection 2022.
2
Enhanced protective efficacy of nonpathogenic recombinant leishmania tarentolae expressing cysteine proteinases combined with a sand fly salivary antigen.表达半胱氨酸蛋白酶的非致病性重组利什曼原虫增强了与沙蝇唾液抗原的保护效力。
PLoS Negl Trop Dis. 2014 Mar 27;8(3):e2751. doi: 10.1371/journal.pntd.0002751. eCollection 2014 Mar.
3
Leishmania tarentolae secreting the sand fly salivary antigen PpSP15 confers protection against Leishmania major infection in a susceptible BALB/c mice model.分泌白蛉唾液抗原PpSP15的大利什曼原虫在易感的BALB/c小鼠模型中可提供针对硕大利什曼原虫感染的保护作用。
Mol Immunol. 2015 Oct;67(2 Pt B):501-11. doi: 10.1016/j.molimm.2015.08.001. Epub 2015 Aug 19.
4
Structural analysis of PpSP15 and PsSP9 sand fly salivary proteins designed with a self-cleavable linker as a live vaccine candidate against cutaneous leishmaniasis.采用自切割连接子设计的 PpSP15 和 PsSP9 沙蝇唾液蛋白的结构分析，作为针对皮肤利什曼病的活疫苗候选物。
Parasit Vectors. 2022 Oct 19;15(1):377. doi: 10.1186/s13071-022-05437-x.
5
DNA plasmid coding for Phlebotomus sergenti salivary protein PsSP9, a member of the SP15 family of proteins, protects against Leishmania tropica.编码白蛉唾液蛋白 PsSP9 的 DNA 质粒，该蛋白属于 SP15 蛋白家族成员，可预防感染利什曼原虫。
PLoS Negl Trop Dis. 2019 Jan 11;13(1):e0007067. doi: 10.1371/journal.pntd.0007067. eCollection 2019 Jan.
6
Immunity to distinct sand fly salivary proteins primes the anti-Leishmania immune response towards protection or exacerbation of disease.对不同沙蝇唾液蛋白的免疫可引发针对利什曼原虫的免疫反应，从而导致疾病的保护或恶化。
PLoS Negl Trop Dis. 2008 Apr 16;2(4):e226. doi: 10.1371/journal.pntd.0000226.
7
Evaluation of a new live recombinant vaccine against cutaneous leishmaniasis in BALB/c mice.评价一种新的针对皮肤利什曼病的活重组疫苗在 BALB/c 小鼠中的效果。
Parasit Vectors. 2020 Aug 12;13(1):415. doi: 10.1186/s13071-020-04289-7.
8
Leishmania major p27 gene knockout as a novel live attenuated vaccine candidate: Protective immunity and efficacy evaluation against cutaneous and visceral leishmaniasis in BALB/c mice.大沙鼠利什曼原虫 p27 基因敲除作为一种新型活减毒疫苗候选物：对 BALB/c 小鼠皮肤和内脏利什曼病的保护免疫和疗效评估。
Vaccine. 2019 May 27;37(24):3221-3228. doi: 10.1016/j.vaccine.2019.04.068. Epub 2019 Apr 29.
9
A Listeria monocytogenes-based vaccine that secretes sand fly salivary protein LJM11 confers long-term protection against vector-transmitted Leishmania major.一种基于李斯特菌的疫苗，可分泌沙蝇唾液蛋白 LJM11，可提供针对媒介传播的利什曼原虫的长期保护。
Infect Immun. 2014 Jul;82(7):2736-45. doi: 10.1128/IAI.01633-14. Epub 2014 Apr 14.
10
Lactococcus lactis expressing sand fly PpSP15 salivary protein confers long-term protection against Leishmania major in BALB/c mice.表达沙蝇 PpSP15 唾液蛋白的乳球菌乳亚种在 BALB/c 小鼠中赋予针对利什曼原虫的长期保护。
PLoS Negl Trop Dis. 2020 Jan 3;14(1):e0007939. doi: 10.1371/journal.pntd.0007939. eCollection 2020 Jan.

引用本文的文献

1
Population structure analysis of Phlebotomus papatasi populations using transcriptome microsatellites: possible implications for leishmaniasis control and vaccine development.利用转录组微卫星进行白蛉埃及亚种种群结构分析：对利什曼病控制和疫苗开发的可能影响。
Parasit Vectors. 2024 Oct 2;17(1):410. doi: 10.1186/s13071-024-06495-z.
2
Live attenuated-nonpathogenic and DNA structures as promising vaccine platforms against leishmaniasis: innovations can make waves.减毒活疫苗-非致病性和DNA结构作为抗利什曼病的有前景的疫苗平台：创新能够引发轰动。
Front Microbiol. 2024 Apr 3;15:1326369. doi: 10.3389/fmicb.2024.1326369. eCollection 2024.
3

本文引用的文献

1
Investigating the Existence of Ribosomal Protein L5 Gene in Syrian Strain of Genome: Sequencing It and Evaluating Its Immune Response as DNA Vaccine.研究叙利亚仓鼠基因组中核糖体蛋白L5基因的存在：对其进行测序并评估其作为DNA疫苗的免疫反应。
J Parasitol Res. 2021 May 20;2021:6617270. doi: 10.1155/2021/6617270. eCollection 2021.
2
Reemergence of zoonotic cutaneous leishmaniasis in an endemic focus, northeastern Iran.伊朗东北部一个地方性疫源地人畜共患皮肤利什曼病再度出现。
Parasite Epidemiol Control. 2021 Feb 3;13:e00206. doi: 10.1016/j.parepi.2021.e00206. eCollection 2021 May.
3
Evaluation of Leishmanization Using Mixed With CpG-ODN as a Candidate Vaccine Against Experimental Murine Leishmaniasis.
Synthetic biology for combating leishmaniasis.
用于对抗利什曼病的合成生物学
Front Microbiol. 2024 Feb 1;15:1338749. doi: 10.3389/fmicb.2024.1338749. eCollection 2024.
4
An overview of the sand fly salivary proteins in vaccine development against leishmaniases.用于抗利什曼病疫苗研发的白蛉唾液蛋白概述。
Iran J Microbiol. 2022 Dec;14(6):792-801. doi: 10.18502/ijm.v14i6.11253.
5
Leishmania tarentolae: a vaccine platform to target dendritic cells and a surrogate pathogen for next generation vaccine research in leishmaniases and viral infections.利什曼原虫：一种以树突状细胞为靶点的疫苗平台，也是针对利什曼病和病毒感染的下一代疫苗研究的替代病原体。
Parasit Vectors. 2023 Jan 26;16(1):35. doi: 10.1186/s13071-023-05651-1.
6
Structural analysis of PpSP15 and PsSP9 sand fly salivary proteins designed with a self-cleavable linker as a live vaccine candidate against cutaneous leishmaniasis.采用自切割连接子设计的 PpSP15 和 PsSP9 沙蝇唾液蛋白的结构分析，作为针对皮肤利什曼病的活疫苗候选物。
Parasit Vectors. 2022 Oct 19;15(1):377. doi: 10.1186/s13071-022-05437-x.
评价混合 CpG-ODN 的利什曼化作为实验性抗小鼠利什曼病候选疫苗。
Front Immunol. 2020 Oct 23;11:1725. doi: 10.3389/fimmu.2020.01725. eCollection 2020.
4
Cloning, high-level gene expression and bioinformatics analysis of SP15 and LeIF from Leishmania major and Iranian Phlebotomus papatasi saliva as single and novel fusion proteins: a potential vaccine candidate against leishmaniasis.SP15 和 LeIF 从利什曼原虫和伊朗白蛉唾液中的克隆、高效基因表达和生物信息学分析：针对利什曼病的单一新型融合蛋白疫苗候选物。
Trans R Soc Trop Med Hyg. 2021 Jun 2;115(6):699-713. doi: 10.1093/trstmh/traa119.
5
Th1 concomitant immune response mediated by IFN-γ protects against sand fly delivered Leishmania infection: Implications for vaccine design.IFN-γ 介导的 Th1 伴随免疫反应可抵抗沙蝇传播的利什曼原虫感染：对疫苗设计的启示。
Cytokine. 2021 Nov;147:155247. doi: 10.1016/j.cyto.2020.155247. Epub 2020 Aug 29.
6
Evaluation of a new live recombinant vaccine against cutaneous leishmaniasis in BALB/c mice.评价一种新的针对皮肤利什曼病的活重组疫苗在 BALB/c 小鼠中的效果。
Parasit Vectors. 2020 Aug 12;13(1):415. doi: 10.1186/s13071-020-04289-7.
7
Lactococcus lactis expressing sand fly PpSP15 salivary protein confers long-term protection against Leishmania major in BALB/c mice.表达沙蝇 PpSP15 唾液蛋白的乳球菌乳亚种在 BALB/c 小鼠中赋予针对利什曼原虫的长期保护。
PLoS Negl Trop Dis. 2020 Jan 3;14(1):e0007939. doi: 10.1371/journal.pntd.0007939. eCollection 2020 Jan.
8
Old world cutaneous leishmaniasis in Iran: clinical variants and treatments.伊朗的旧世界皮肤利什曼病：临床变种与治疗方法
J Dermatolog Treat. 2021 Nov;32(7):673-683. doi: 10.1080/09546634.2019.1704214. Epub 2020 Jan 8.
9
Leishmania major p27 gene knockout as a novel live attenuated vaccine candidate: Protective immunity and efficacy evaluation against cutaneous and visceral leishmaniasis in BALB/c mice.大沙鼠利什曼原虫 p27 基因敲除作为一种新型活减毒疫苗候选物：对 BALB/c 小鼠皮肤和内脏利什曼病的保护免疫和疗效评估。
Vaccine. 2019 May 27;37(24):3221-3228. doi: 10.1016/j.vaccine.2019.04.068. Epub 2019 Apr 29.
10
DNA plasmid coding for Phlebotomus sergenti salivary protein PsSP9, a member of the SP15 family of proteins, protects against Leishmania tropica.编码白蛉唾液蛋白 PsSP9 的 DNA 质粒，该蛋白属于 SP15 蛋白家族成员，可预防感染利什曼原虫。
PLoS Negl Trop Dis. 2019 Jan 11;13(1):e0007067. doi: 10.1371/journal.pntd.0007067. eCollection 2019 Jan.