Liu Nian, Wang Hui, Fu Zeyu, Zhang Chuxi, Hui Wenyu, Wu Jinyang, Zhang Yong, Zhang Shilei
Department of Oral and Cranio-Maxillofacial Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, Shanghai, China.
School of Materials and Chemistry, University of Shanghai for Science and Technology, Shanghai, China.
Front Bioeng Biotechnol. 2022 Jun 8;10:933135. doi: 10.3389/fbioe.2022.933135. eCollection 2022.
The capabilities of osseointegration and anti-inflammatory properties are of equal significance to the bio-inert titanium implant surface. Quercetin has proved its capacities of activating anti-inflammation through macrophage modulation and promoting osteogenic differentiation. Herein, we fabricated quercetin-coating on nano-topographic modificated 3D-printed Ti6Al4V implant surface. Subsequently the biological cells responses , anti-inflammatory and osseointegration performance were evaluated. studies indicated that quercetin-coating can enhance the adhesion and osteogenic differentiation of rBMSCs, while modulating the polarization of macrophages from M1 to M2 phase and improving the anti-inflammatory and vascular gene expression. Moreover, quercetin-loaded implants reduced the level of peri-implant inflammation and ameliorated new bone formation and rapid osseoinegration . Quercetin-coating might provide a feasible and favorable scheme for endowing 3D-printed titanium alloy implant surface with enhanced the rapid osseointegration and anti-inflammatory properties.
骨整合能力和抗炎特性对于生物惰性钛植入物表面同样重要。槲皮素已证明其具有通过巨噬细胞调节激活抗炎作用以及促进成骨分化的能力。在此，我们在纳米拓扑修饰的3D打印Ti6Al4V植入物表面制备了槲皮素涂层。随后评估了生物细胞反应、抗炎和骨整合性能。研究表明，槲皮素涂层可增强rBMSCs的黏附和成骨分化，同时调节巨噬细胞从M1期向M2期的极化，并改善抗炎和血管基因表达。此外，载有槲皮素的植入物降低了植入物周围的炎症水平，改善了新骨形成和快速骨整合。槲皮素涂层可能为赋予3D打印钛合金植入物表面增强的快速骨整合和抗炎特性提供一种可行且有利的方案。
