Wang Yijin, Jin Yabing, Chen Yiyi, Han Tianlei, Chen Yuhong, Wang Chen
Jiangsu Key Laboratory of Oral Diseases, Department of Prosthodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China.
J Biomater Sci Polym Ed. 2022 Oct;33(14):1845-1865. doi: 10.1080/09205063.2022.2088524. Epub 2022 Jun 26.
Polyaryletherketone (PAEK) has good biocompatibility and mechanical properties and thus may have great potential in the fields of reparative medicine and bone intervention. In this study, the key representative PAEKs, polyetheretherketone (PEEK) and polyetherketoneketone (PEKK), were modified by UV grafting with sodium polystyrene sulfonate (polyNaSS) to improve their biocompatibility. Toluidine blue staining and Fourier transform infrared spectroscopic analyses showed that sulfonic acid groups were successfully introduced into PAEK, and the hydrophilicity and protein adsorption capacity of the materials were enhanced in a concentration-dependent manner. The effects of the grafted polyNaSS on osteoinduction and antibacterial properties of PAEK were analyzed in detail. We found that polyNaSS enhanced the viability, alkaline phosphatase activity, calcium mineral deposition, and levels of expression of osteoblast-related genes and proteins of adherent human umbilical cord Wharton's jelly-derived mesenchymal stem cells. In addition, when , and were incubated with the materials, bacterial colony counting revealed that grafting of polyNaSS onto PAEK led to more potent inhibition of bacterial adhesion, and polyNaSS-grafted PEKK had stronger antibacterial performance than did polyNaSS-grafted PEEK fabricated under the same grafting conditions. These data show that polyNaSS-grafted PAEK, and particularly polyNaSS-grafted PEKK, may be useful as orthopedic and dental implant materials.
聚芳醚酮（PAEK）具有良好的生物相容性和机械性能，因此在修复医学和骨干预领域可能具有巨大潜力。在本研究中，通过用聚苯乙烯磺酸钠（聚NaSS）进行紫外接枝对关键代表性PAEKs，聚醚醚酮（PEEK）和聚醚酮酮（PEKK）进行改性，以提高它们的生物相容性。甲苯胺蓝染色和傅里叶变换红外光谱分析表明磺酸基团成功引入到PAEK中，并且材料的亲水性和蛋白质吸附能力以浓度依赖的方式增强。详细分析了接枝的聚NaSS对PAEK的骨诱导和抗菌性能的影响。我们发现聚NaSS提高了贴壁的人脐带华通氏胶源性间充质干细胞的活力、碱性磷酸酶活性、钙矿物质沉积以及成骨细胞相关基因和蛋白质的表达水平。此外，当与材料一起孵育时，细菌菌落计数显示聚NaSS接枝到PAEK上导致对细菌粘附的更强抑制，并且在相同接枝条件下制备的聚NaSS接枝的PEKK比聚NaSS接枝的PEEK具有更强的抗菌性能。这些数据表明聚NaSS接枝的PAEK，特别是聚NaSS接枝的PEKK，可能用作骨科和牙科植入材料。
