Yao Yanling, Zhang Xiandan, Xu Yixuan, Zhao Yimin, Song Fenglin, Tian Zezhong, Zhao Mingzhu, Liang Ying, Ling Wenhua, Mao Yu-Heng, Yang Yan
School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Sun Yat-Sen University, Shenzhen, Guangdong Province 518107, China.
Guangdong Provincial Key Laboratory for Food, Nutrition and Health, Guangzhou, Guangdong Province 510080, China.
J Agric Food Chem. 2022 Jul 13;70(27):8254-8263. doi: 10.1021/acs.jafc.2c01844. Epub 2022 Jun 27.
Platelet chemokines play well-established roles in the atherosclerotic inflammation. Cyanidin-3--β-glucoside (Cy-3-g) is one of the main bioactive compounds in anthocyanins, but its effects on chemokines during atherosclerosis have not been determined yet. In the present study, ApoE mice were fed on the chow diet, high-fat diet (HFD), and HFD-supplemented Cy-3-g at 200, 400, and 800 mg/kg diet. After 16 weeks, Cy-3-g significantly alleviated the atherosclerotic lesion and inhibited platelet aggregation and activation. Moreover, Cy-3-g significantly reduced inflammatory chemokines CXCL4, CXCL7, CCL5, CXCL5, CXCL12, and CCL2 in plasma and downregulated CXCR4, CXCR7, and CCR5 on platelets and peripheral blood mononuclear cells. Besides, Cy-3-g decreased the mRNA of TNFα, IFNγ, ICAM-1, VCAM-1, CD68, MMP7, CCL5, CXCR4, and CCR5 in the aorta of mice. Therefore, it suggests that Cy-3-g plays important preventive roles in the process of atherosclerosis via attenuating chemokines and receptors in ApoE mice.
血小板趋化因子在动脉粥样硬化炎症中发挥着既定作用。矢车菊素 - 3 - β - 葡萄糖苷（Cy - 3 - g）是花青素中的主要生物活性化合物之一，但其在动脉粥样硬化过程中对趋化因子的影响尚未确定。在本研究中，给载脂蛋白E（ApoE）小鼠喂食普通饲料、高脂饮食（HFD）以及添加了200、400和800毫克/千克Cy - 3 - g的高脂饮食。16周后，Cy - 3 - g显著减轻了动脉粥样硬化病变，并抑制了血小板聚集和活化。此外，Cy - 3 - g显著降低了血浆中炎性趋化因子CXCL4、CXCL7、CCL5、CXCL5、CXCL12和CCL2的水平，并下调了血小板和外周血单核细胞上的CXCR4、CXCR7和CCR5。此外，Cy - 3 - g降低了小鼠主动脉中TNFα、IFNγ、ICAM - 1、VCAM - 1、CD68、MMP7、CCL5、CXCR4和CCR5的mRNA水平。因此，这表明Cy - 3 - g通过减弱ApoE小鼠中的趋化因子和受体，在动脉粥样硬化过程中发挥重要的预防作用。
