Zhang Zhe, Chen Yun, Li Xinpu, Wang Xurong, Li Hongsheng
Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences/KeyLab of Veterinary Pharmaceutical Development, Ministry of Agriculture and Rural Affairs/Engineering and Technology Research Center of Traditional Chinese Veterinary Medicine, Lanzhou, People's Republic of China.
Microbiol Spectr. 2022 Aug 31;10(4):e0047122. doi: 10.1128/spectrum.00471-22. Epub 2022 Jun 27.
Antimicrobial therapy plays an important role in mastitis control caused by Staphylococcus aureus but has become less effective due to widespread drug resistance. The purpose of this study was to detect antibiotic resistance, drug resistance gene, and virulence gene of S. aureus strains. In this study, 2,962 milk samples were collected from 43 dairy farms located in 16 provinces of China and cultured for isolation of S. aureus. Antibiotic resistance, capsular polysaccharide, spa typing, virulence genes, and drug resistance genes of the strains were analyzed. Of 2,962 samples, 298 strains were isolated and identified as S. aureus. The strains exhibited high percentages of resistance to penicillin G (91.95%). Moreover, all strains showed resistance to more than one antimicrobial agent but were sensitive to nitrofurantoin and sulfamethoxazole/trimethoprim. The results indicate that type 8 was the dominant capsular polysaccharide serotype and t459 was the dominant spa type. The most prevalent virulence gene was (98%). The resistance genes of several antibiotics were detected, among which the gene (92.95%) was the highest. In conclusion, we present the antimicrobial resistance and virulence genes of S. aureus in this study which are of importance for mastitis control. Bovine mastitis is a serious disease associated with both high incidence and economic loss, posing a major challenge to the dairy industry worldwide. Staphylococcus aureus is one of the most common pathogens to cause bovine mastitis, and antimicrobial therapy plays an important role in mastitis control caused by S. aureus but has become less effective due to widespread drug resistance. The purpose of this study was to detect antibiotic resistance, drug resistance gene, and virulence gene of S. aureus strains, which would be helpful to mastitis control.
抗菌治疗在由金黄色葡萄球菌引起的乳腺炎防控中发挥着重要作用，但由于广泛的耐药性，其效果已逐渐降低。本研究的目的是检测金黄色葡萄球菌菌株的抗生素耐药性、耐药基因和毒力基因。在本研究中，从中国16个省份的43个奶牛场采集了2962份牛奶样本，进行培养以分离金黄色葡萄球菌。对分离菌株的抗生素耐药性、荚膜多糖、spa分型、毒力基因和耐药基因进行了分析。在2962份样本中，分离并鉴定出298株金黄色葡萄球菌。这些菌株对青霉素G的耐药率很高（91.95%）。此外，所有菌株对不止一种抗菌药物耐药，但对呋喃妥因和磺胺甲恶唑/甲氧苄啶敏感。结果表明，8型是主要的荚膜多糖血清型，t459是主要的spa型。最普遍的毒力基因是（98%）。检测到了几种抗生素的耐药基因，其中基因（92.95%）的检出率最高。总之，我们在本研究中呈现了金黄色葡萄球菌的抗菌耐药性和毒力基因，这对乳腺炎的防控具有重要意义。牛乳腺炎是一种发病率高且造成经济损失的严重疾病，对全球乳制品行业构成重大挑战。金黄色葡萄球菌是引起牛乳腺炎最常见的病原体之一，抗菌治疗在由金黄色葡萄球菌引起的乳腺炎防控中发挥着重要作用，但由于广泛的耐药性，其效果已逐渐降低。本研究的目的是检测金黄色葡萄球菌菌株的抗生素耐药性、耐药基因和毒力基因，这将有助于乳腺炎的防控。
