Department of Biology, Laboratory of Postgraduates, College of Science, University of Kerbala, Karbala, Iraq.
Department of Chemistry, Laboratory of Postgraduates, College of Science, University of Kerbala, Karbala, Iraq.
Egypt J Immunol. 2022 Jul;29(3):1-8.
Aerobic bacteria can colonize the female reproductive system with harmful effects, which may lead to miscarriages, premature deliveries or continue of its growing to cause adverse reproductive systems issues. Increasing in the levels of inflammatory markers may be considered a herald of danger. High vaginal swabs were obtained from 85 women. Of these, 67 patients were suffering recurrent vaginitis and symptoms such as itching, irritation, burning, and vaginal discharged, and 18 apparently healthy controls. Swabs were cultured in a suitable media and the cultivated bacteria were diagnosed in the hospital's laboratory. At the same time of collecting the vaginal swabs, 5 ml of venous blood was withdrawn from the patients and controls. An ELISA method was applied to measure the levels of inflammatory cytokines and concentration of vitamin D. The bacterial growth showed six species of isolated bacteria, which were Staphylococcus aureus, Streptococcus spp., E. coli, S. non aureus, Proteus spp. and Klebsiella spp. The first three species were the most prevalent bacteria, and the serum levels of C reactive protein (CRP) and IL-6 were high in female patients infected with these bacteria. CRP was significantly elevated in sera of the patient's group (P= 0.016), while the increase in IL-6 was not significant. Vitamin D was correlated negatively with IL-6 and positively with CRP, but the correlations did not reach statistical significance. In conclusion, rising of CRP could be an expected result to the bacterial colonizing the reproductive system while IL-6 may develop significantly when the aerobic vaginitis continues until triggering one of the infertility issues.
需氧菌可定植于女性生殖系统，产生有害影响，导致流产、早产或继续生长，引起不良的生殖系统问题。炎症标志物水平升高可能被视为危险的先兆。从 85 名女性中采集了阴道拭子。其中，67 名患者患有复发性阴道炎，出现瘙痒、刺激、烧灼感和阴道分泌物等症状，18 名明显健康的对照组。将拭子接种于合适的培养基中，在医院实验室中诊断培养的细菌。在采集阴道拭子的同时，从患者和对照组抽取 5 毫升静脉血。应用 ELISA 法测量炎症细胞因子水平和维生素 D 浓度。细菌生长显示分离出六种细菌，分别为金黄色葡萄球菌、链球菌属、大肠杆菌、非金黄色葡萄球菌、变形杆菌属和克雷伯菌属。前三种细菌是最常见的细菌，感染这些细菌的女性患者血清 C 反应蛋白（CRP）和白细胞介素 6（IL-6）水平较高。患者组血清 CRP 显著升高（P=0.016），而 IL-6 升高不显著。维生素 D 与 IL-6 呈负相关，与 CRP 呈正相关，但相关性没有达到统计学意义。总之，CRP 的升高可能是细菌定植于生殖系统的预期结果，而当需氧性阴道炎持续发展，导致其中一种不孕问题时，IL-6 可能会显著升高。
