Faculty of Pharmacy, Department of Pharmaceutical Microbiology, Ankara University, Ankara, Turkey.
Turkish Medicines and Medical Devices Agency, Ankara, Turkey.
Arch Microbiol. 2022 Jun 27;204(7):432. doi: 10.1007/s00203-022-03000-x.
Honeybee products have been among important consumer products throughout history. Microbiota has attracted attention in recent years due to both their probiotic value and industrial potential. Fructophilic lactic acid bacteria (FLAB), whose field of study has been expanding rapidly in the last 20 years, are among the groups that can be isolated from the bee gut. This study aimed to isolate FLAB from the honeybees of two different geographic regions in Turkey and investigate their probiotic, metabolic and anti-quorum sensing (anti-QS) potential. Metabolic properties were investigated based on fructose toleration and acid and diacetyl production while the probiotic properties of the isolates were determined by examining pH, pepsin, pancreatin resistance, antimicrobial susceptibility, and antimicrobial activity. Anti-QS activities were also evaluated with the Chromobacterium violaceum biosensor strain. Two FLAB members were isolated and identified by the 16S rRNA analysis as Fructobacillus tropaeoli and Apilactobacillus kunkeei, which were found to be tolerant to high fructose, low pH, pepsin, pancreatin, and bile salt environments. Both isolates showed anti-QS activity against the C. violaceum biosensor strain and no diacetyl production. The daily supernatants of the isolates inhibited the growth of Enterococcus faecalis ATCC 29212 among the selected pathogens. The isolates were found resistant to kanamycin, streptomycin, erythromycin, and clindamycin. In the evaluation of the probiotic potential of these species, the negative effect of antibiotics and other chemicals to which honeybees are directly or indirectly exposed draws attention within the scope of the "One Health" approach.
蜂蜜产品一直是历史上重要的消费品之一。由于其益生菌价值和工业潜力，近年来微生物群受到了关注。在过去的 20 年中，其研究领域迅速扩展的嗜果糖乳酸菌（FLAB）是可以从蜜蜂肠道中分离出来的菌群之一。本研究旨在从土耳其两个不同地理区域的蜜蜂中分离 FLAB，并研究它们的益生菌、代谢和抗群体感应（anti-QS）潜力。代谢特性是基于果糖耐受性和酸和二乙酰的产生来研究的，而分离株的益生菌特性则通过检查 pH 值、胃蛋白酶、胰蛋白酶抗性、抗菌敏感性和抗菌活性来确定。还使用 Chromobacterium violaceum 生物传感器菌株评估了 anti-QS 活性。通过 16S rRNA 分析，从两种 FLAB 成员中分离并鉴定出 Fructobacillus tropaeoli 和 Apilactobacillus kunkeei，它们被发现能够耐受高果糖、低 pH 值、胃蛋白酶、胰蛋白酶和胆盐环境。两种分离株均对 C. violaceum 生物传感器菌株表现出 anti-QS 活性，且不产生二乙酰。分离株的每日上清液抑制了所选病原体中粪肠球菌 ATCC 29212 的生长。分离株对卡那霉素、链霉素、红霉素和克林霉素具有抗性。在评估这些物种的益生菌潜力时，抗生素和其他蜜蜂直接或间接暴露的化学物质的负面影响引起了人们对“One Health”方法的关注。
