Todorovic Dusan, Stojanovic Marija, Gopcevic Kristina, Medic Ana, Stankovic Sanja, Kotlica Boba, Labudovic Borovic Milica, Djuric Dragan
Institute of Medical Physiology "Richard Burian", Faculty of Medicine, University of Belgrade, Visegradska 26/II, 11000, Belgrade, Serbia.
Institute of Chemistry in Medicine "Prof. Dr. Petar Matavulj", Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
Mol Cell Biochem. 2023 Jan;478(1):161-172. doi: 10.1007/s11010-022-04503-3. Epub 2022 Jun 27.
The aim of this study was to examine the effects of hyperhomocysteinemia and aerobic physical activity on changes of cardiovascular biomarkers in sera, oxidative stress in cardiac tissue, and histomorphometric parameters of heart and aorta in rats. Experiments were conducted on male Wistar albino rats organized into four groups (n = 10, per group): C (control group): 0.9% NaCl 0.2 mL/day; H (homocysteine group): homocysteine 0.45 µmol/g b.w./day; CPA (control + physical activity group): 0.9% NaCl 0.2 mL/day and a program of physical activity on a treadmill; and HPA (homocysteine + physical activity group) homocysteine 0.45 µmol/g b.w./day and a program of physical activity on a treadmill. Substances were applied subcutaneously twice a day. Lipid peroxidation and relative activity of Mn-superoxide dismutase isoform were significantly higher in active hyperhomocysteinemic rats in comparison to sedentary animals. Atherosclerotic plaques were detected in aorta samples of active hyperhomocysteinemic rats and also, they had increased left ventricle wall and interventricular septum, and transverse diameter of cardiomyocytes compared to sedentary groups. Aerobic physical activity in the condition of hyperhomocysteinemia can lead to increased oxidative stress in cardiac tissue and changes in histomorphometric parameters of the heart and aorta, as well increased lipid parameters and cardiac damage biomarkers in sera of rats.
本研究旨在探讨高同型半胱氨酸血症和有氧运动对大鼠血清中心血管生物标志物变化、心脏组织氧化应激以及心脏和主动脉组织形态计量学参数的影响。实验选用雄性Wistar白化大鼠，分为四组（每组n = 10）：C组（对照组）：每天皮下注射0.2 mL 0.9%氯化钠溶液；H组（同型半胱氨酸组）：每天皮下注射同型半胱氨酸0.45 μmol/g体重；CPA组（对照组 + 运动组）：每天皮下注射0.2 mL 0.9%氯化钠溶液，并进行跑步机运动；HPA组（同型半胱氨酸 + 运动组）：每天皮下注射同型半胱氨酸0.45 μmol/g体重，并进行跑步机运动。药物每天皮下注射两次。与久坐不动的动物相比，活跃的高同型半胱氨酸血症大鼠的脂质过氧化和锰超氧化物歧化酶同工型的相对活性显著更高。在活跃的高同型半胱氨酸血症大鼠的主动脉样本中检测到动脉粥样硬化斑块，并且与久坐组相比，它们的左心室壁、室间隔以及心肌细胞横径均增加。在高同型半胱氨酸血症状态下进行有氧运动可导致心脏组织氧化应激增加、心脏和主动脉组织形态计量学参数改变，以及大鼠血清中脂质参数和心脏损伤生物标志物增加。
