Todorović Dušan, Stojanović Marija, Mutavdžin Krneta Slavica, Jakovljević Uzelac Jovana, Gopčević Kristina, Medić Ana, Labudović Borović Milica, Stanković Sanja, Djuric Dragan M
Faculty of Medicine, Institute of Medical Physiology "Richard Burian", University of Belgrade, Belgrade, Serbia.
Faculty of Medicine, Institute of Chemistry in Medicine "Petar Matavulj", University of Belgrade, Belgrade, Serbia.
Mol Cell Biochem. 2025 Apr;480(4):2511-2524. doi: 10.1007/s11010-024-05133-7. Epub 2024 Oct 10.
Disruptions in homocysteine (Hcy) metabolism may increase the liver's susceptibility to developing conditions such as alcoholic liver disease, viral hepatitis, hepatocellular carcinoma (HCC), and cirrhosis. The aim of this study was to examine effects of aerobic treadmill training on hepatic injury biomarkers in sera, oxidative stress parameters, the activity of metabolic enzymes, and histological characteristics in the liver tissue of rats with experimentally induced hyperhomocysteinemia. Male Wistar albino rats were divided into four groups (N = 10, per group): C-saline 0.2 mL/day sc. 2×/day for 14 days + saline 0.5 mL ip.1×/day for 28 days; H-homocysteine 0.45 µmol/g b.w. 2×/day for 14 days + saline 0.5 mL ip.1×/day for 28 days; CPA-saline 0.2 mL/day sc. 2×/day for 14 days + aerobic treadmill training for 28 days; and HPA-homocysteine 0.45 µmol/g b.w. 2×/day for 14 days + aerobic treadmill training for 28 days. The serum albumin concentration was decreased in both physically active (PA) groups compared to sedentary groups. Concentration of malondialdehyde in liver tissue homogenates was lower in both PA groups compared to the H group. The total lactate dehydrogenase and malate dehydrogenase activities were significantly elevated in the HPA group compared to the C and H groups. Activities of aminotransferases in sera samples, and activities of catalase and superoxide dismutase in liver tissue did not significantly differ between groups. No significant histological changes were found in liver tissue in groups. This study demonstrated that aerobic treadmill training can reduce lipid peroxidation in liver tissue under hyperhomocysteinemic conditions, providing a protective effect. However, hyperhomocysteinemia can alter energy metabolism during aerobic exercise, shifting it toward anaerobic pathways and leading to elevated lactate dehydrogenase activity in the liver. Given that conditions like hyperhomocysteinemia are associated with an increased risk of cardiovascular diseases and liver damage, understanding how exercise influences these dynamics could guide therapeutic approaches.
同型半胱氨酸（Hcy）代谢紊乱可能会增加肝脏患酒精性肝病、病毒性肝炎、肝细胞癌（HCC）和肝硬化等疾病的易感性。本研究的目的是探讨有氧跑步机训练对实验性诱导的高同型半胱氨酸血症大鼠血清中肝损伤生物标志物、氧化应激参数、代谢酶活性以及肝组织组织学特征的影响。雄性Wistar白化大鼠分为四组（每组N = 10）：C组，皮下注射0.2 mL/天生理盐水，每天2次，共14天，然后腹腔注射0.5 mL生理盐水，每天1次，共28天；H组，皮下注射0.45 μmol/g体重的同型半胱氨酸，每天2次，共14天，然后腹腔注射0.5 mL生理盐水，每天1次，共28天；CPA组，皮下注射0.2 mL/天生理盐水，每天2次，共14天，然后进行28天的有氧跑步机训练；HPA组，皮下注射0.45 μmol/g体重的同型半胱氨酸，每天2次，共14天，然后进行28天的有氧跑步机训练。与久坐组相比，两个运动组的血清白蛋白浓度均降低。与H组相比，两个运动组肝组织匀浆中丙二醛的浓度均较低。与C组和H组相比，HPA组的总乳酸脱氢酶和苹果酸脱氢酶活性显著升高。各组血清样本中氨基转移酶的活性以及肝组织中过氧化氢酶和超氧化物歧化酶的活性无显著差异。各组肝组织未发现明显的组织学变化。本研究表明，有氧跑步机训练可在高同型半胱氨酸血症条件下降低肝组织中的脂质过氧化，起到保护作用。然而，高同型半胱氨酸血症可改变有氧运动期间的能量代谢，使其转向无氧途径，并导致肝脏中乳酸脱氢酶活性升高。鉴于高同型半胱氨酸血症等情况与心血管疾病和肝损伤风险增加有关，了解运动如何影响这些动态变化可为治疗方法提供指导。
