Xu Ruoxin, Xie Siming, Gong Ju, Chen Wei, Jin Yakang, Huang Jian
Department of Critical Care Medicine, The First Affiliated Hospital of Suzhou University, No.188 Shizi Street, Suzhou, 215000, Jiangsu, China.
Department of Emergency, Changshu No. 2 People's Hospital, Suzhou, Jiangsu, China.
Dig Dis Sci. 2023 Mar;68(3):824-830. doi: 10.1007/s10620-022-07585-7. Epub 2022 Jun 27.
B7-H5 is an important ligand which is deeply involved in the immune response in various diseases. However, its clinical usefulness as an early indicator in acute pancreatitis (AP) remains unclear.
To determine the role of B7-H5 in severe acute pancreatitis (SAP).
Whole blood samples from patients with SAP (n = 20) and healthy donors (n = 20) were collected. Expression of soluble B7-H5 (sB7-H5) in plasma was determined by ELISA and membrane B7-H5 (mB7-H5) on the peripheral CD14+ cells was determined by flow cytometry. Peripheral blood mononuclear cells (PBMCs) were isolated from healthy donors and stimulated with serum from SAP patients, lipopolysaccharide (LPS), TNF-α, or IFN-γ, then, sB7-H5 and mB7-H5 were measured. The relationship between expression levels of mB7-H5 and clinical features of SAP patients were analyzed.
The expression levels of sB7-H5 in plasma were increased and the expression levels of mB7-H5 on the peripheral CD14+ cells were decreased in SAP patients. These changes of B7-H5 expression pattern in cultured PBMCs could be induced by stimulation with serum from SAP patients, LPS, TNF-α, or IFN-γ. Expression levels of mB7-H5 were negatively related to levels of hematocrit, urea nitrogen, creatinine, lactic acid, RANSON scores, and APACHE II scores.
Changes of B7-H5 expression pattern were involved in immune response of SAP. Innate immunity activation-induced decrease of mB7-H5 might be related to poor prognosis of SAP patients.
B7-H5是一种重要的配体，深度参与多种疾病的免疫反应。然而，其作为急性胰腺炎（AP）早期指标的临床实用性仍不明确。
确定B7-H5在重症急性胰腺炎（SAP）中的作用。
收集SAP患者（n = 20）和健康供者（n = 20）的全血样本。采用酶联免疫吸附测定法（ELISA）测定血浆中可溶性B7-H5（sB7-H5）的表达，采用流式细胞术测定外周血CD14+细胞上的膜B7-H5（mB7-H5）。从健康供者中分离外周血单个核细胞（PBMCs），并用SAP患者的血清、脂多糖（LPS）、肿瘤坏死因子-α（TNF-α）或干扰素-γ（IFN-γ）进行刺激，然后测定sB7-H5和mB7-H5。分析mB7-H5表达水平与SAP患者临床特征之间的关系。
SAP患者血浆中sB7-H5的表达水平升高，外周血CD14+细胞上mB7-H5的表达水平降低。SAP患者血清、LPS、TNF-α或IFN-γ刺激可诱导培养的PBMCs中B7-H5表达模式发生这些变化。mB7-H5的表达水平与血细胞比容、尿素氮、肌酐、乳酸、RANSON评分和APACHE II评分呈负相关。
B7-H5表达模式的变化参与了SAP的免疫反应。天然免疫激活诱导的mB7-H5降低可能与SAP患者预后不良有关。
